10.1371/journal.pone.0057128.g005 Leticia V. Bentancor Leticia V. Bentancor Marcos F. Bilen Marcos F. Bilen María P. Mejías María P. Mejías Romina J. Fernández-Brando Romina J. Fernández-Brando Cecilia A. Panek Cecilia A. Panek Maria V. Ramos Maria V. Ramos Gabriela C. Fernández Gabriela C. Fernández Martín Isturiz Martín Isturiz Pablo D. Ghiringhelli Pablo D. Ghiringhelli Marina S. Palermo Marina S. Palermo Immunofluorescence assay of Vero cells incubated with Stx2. Public Library of Science 2013 assay vero cells incubated 2013-02-23 11:28:26 Figure https://plos.figshare.com/articles/figure/_Immunofluorescence_assay_of_Vero_cells_incubated_with_Stx2_/638318 <p>Cells were incubated with purified Stx2 (100 CD50) or transfected with the pStx2 plasmid and stained with propidium iodide. Presence of Stx2 was detected with polyclonal anti-Stx2 serum and FITC-conjugated goat anti-mouse antibody, and analyzed by confocal microscopy. Original magnification 600X. <b>A:</b> Non-treated Vero cells. <b>B:</b> Cells transfected with the pGEM-T vector alone. <b>C:</b> Cells incubated with purified Stx2. <b>D:</b> Digital zoom (3.5X) of picture C. <b>E:</b> Cells transfected with pStx2. <b>F:</b> Digital zoom (3.5X) of picture <b>E</b>.</p>