10.1371/journal.pone.0057128.g005
Leticia V. Bentancor
Leticia
V. Bentancor
Marcos F. Bilen
Marcos
F. Bilen
María P. Mejías
María
P. Mejías
Romina J. Fernández-Brando
Romina
J. Fernández-Brando
Cecilia A. Panek
Cecilia
A. Panek
Maria V. Ramos
Maria
V. Ramos
Gabriela C. Fernández
Gabriela
C. Fernández
Martín Isturiz
Martín
Isturiz
Pablo D. Ghiringhelli
Pablo
D. Ghiringhelli
Marina S. Palermo
Marina
S. Palermo
Immunofluorescence assay of Vero cells incubated with Stx2.
Public Library of Science
2013
assay
vero
cells
incubated
2013-02-23 11:28:26
Figure
https://plos.figshare.com/articles/figure/_Immunofluorescence_assay_of_Vero_cells_incubated_with_Stx2_/638318
<p>Cells were incubated with purified Stx2 (100 CD50) or transfected with the pStx2 plasmid and stained with propidium iodide. Presence of Stx2 was detected with polyclonal anti-Stx2 serum and FITC-conjugated goat anti-mouse antibody, and analyzed by confocal microscopy. Original magnification 600X. <b>A:</b> Non-treated Vero cells. <b>B:</b> Cells transfected with the pGEM-T vector alone. <b>C:</b> Cells incubated with purified Stx2. <b>D:</b> Digital zoom (3.5X) of picture C. <b>E:</b> Cells transfected with pStx2. <b>F:</b> Digital zoom (3.5X) of picture <b>E</b>.</p>