EAK-4 Amino Acid Sequence and Alignment with Four C. elegans Homologs Patrick J Hu Jinling Xu Gary Ruvkun 10.1371/journal.pgen.0020099.g003 https://plos.figshare.com/articles/figure/_EAK_4_Amino_Acid_Sequence_and_Alignment_with_Four_C_elegans_Homologs_/628485 <p>Sequences represent the entire predicted amino acid sequences of all five genes. EAK-4 sequence was derived from full-length cDNA amplified by RT-PCR from wild-type C. elegans total RNA. Alignment was constructed using ClustalX 1.8 and MacBoxshade 2.15. Shaded residues indicate amino acid identity and conservation. Mutated residues in three alleles of <i>eak-4</i> are boxed, and the predicted amino acid change is indicated above the box. The conserved G at residue 2 and S/T/A (boxed) at residue 6 in the <i>N</i>-myristoylation consensus sequence [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020099#pgen-0020099-b070" target="_blank">70</a>] are denoted by an asterisk and a vertical arrow, respectively.</p> 2013-02-22 08:35:41 amino alignment