Wellmer, Frank Alves-Ferreira, Márcio Dubois, Annick Luis Riechmann, José M Meyerowitz, Elliot Microarray Results for Selected Floral Regulatory Genes <div><p>Log<sub>10</sub>-transformed signal intensities at the individual time points of the experiment are shown.</p><p>(A) Expression dynamics of previously identified AP1 response genes that regulate the initiation of flower formation (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020117#pgen-0020117-g001" target="_blank">Figure 1</a>). The floral meristem identity gene <i>LEAFY (LFY)</i> is rapidly upregulated upon AP1-GR activation, whereas <i>TERMINAL FLOWER1 (TFL1)</i> and <i>AGAMOUS-LIKE 24 (AGL24)</i> are repressed. The slight reduction in expression of <i>FRUITFULL (FUL)</i> was not judged statistically significant in our analysis.</p><p>(B) Repression of <i>AGAMOUS-LIKE42 (AGL42), SHORT VEGETATIVE PHASE (SVP),</i> and <i>SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1).</i></p><p>(C) Expression dynamics of genes involved in specifying the identity of floral organs. <i>AP3</i>: <i>APETALA3</i>; <i>PI</i>: <i>PISTILLATA</i>; <i>AG</i>: <i>AGAMOUS</i>; <i>SEP3</i>: <i>SEPALLATA3</i>; <i>AP2</i>: <i>APETALA2</i>.</p><p>(D) Activation of genes involved in floral patterning <i>(SUP: SUPERMAN)</i> or organ primordia formation <i>(RBE: RABBIT EARS; PRS: PRESSED FLOWERS).</i></p><p>(E) Induction of genes involved in carpel or stamen primordia development was detected towards the end of the time course experiment <i>(SHP1</i>: <i>SHATTERPROOF1</i>; <i>SHP2</i>: <i>SHATTERPROOF2</i>; <i>CRC</i>: <i>CRABS CLAW</i>; <i>NZZ/SPL</i>: <i>NOZZLE/SPOROCYTELESS).</i> Expression of <i>SHP1</i> was not judged significantly changed in the experiment in contrast to that of its paralog <i>SHP2</i>. This result is in agreement with the reported induction of <i>SHP2</i> at stage 6, one stage earlier than that of <i>SHP1</i> [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020117#pgen-0020117-b061" target="_blank">61</a>,<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020117#pgen-0020117-b062" target="_blank">62</a>].</p><p>(F) The meristem regulatory genes <i>WUSCHEL (WUS)</i> and <i>CLAVATA3 (CLV3)</i> were gradually downregulated during the course of the experiment. The increase in <i>WUS</i> expression on day 5 likely marks the onset of its expression in stamen primordia [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020117#pgen-0020117-b016" target="_blank">16</a>].</p><p>(G) Similar expression profiles (correlation coefficient of 0.84) were observed for <i>FILAMENTOUS FLOWER (FIL)</i> and <i>YABBY3 (YAB3)</i> in agreement with their largely identical expression patterns in developing flowers [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020117#pgen-0020117-b029" target="_blank">29</a>].</p><p>(H) Co-expression of <i>JAGGED (JAG)</i> and its paralog <i>NUBBIN (NUB).</i> Gene identifiers and references are listed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.0020117#pgen-0020117-st001" target="_blank">Table S1</a>.</p></div> floral 2013-02-22
    https://plos.figshare.com/articles/figure/_Microarray_Results_for_Selected_Floral_Regulatory_Genes_/628484
10.1371/journal.pgen.0020117.g004