10.1371/journal.pbio.0040261.g004 Elena O Gracheva Elena O Gracheva Anna O Burdina Anna O Burdina Andrea M Holgado Andrea M Holgado Martine Berthelot-Grosjean Martine Berthelot-Grosjean Brian D Ackley Brian D Ackley Gayla Hadwiger Gayla Hadwiger Michael L Nonet Michael L Nonet Robby M Weimer Robby M Weimer Janet E Richmond Janet E Richmond tom-1(ok285) Mutants Accumulate Vesicles That Are Contacting the Plasma Membrane Public Library of Science 2013 mutants accumulate vesicles contacting plasma 2013-02-22 08:35:39 Figure https://plos.figshare.com/articles/figure/_tom_1_ok285_Mutants_Accumulate_Vesicles_That_Are_Contacting_the_Plasma_Membrane_/628457 <div><p>(A) Examples of synaptic profiles from WT and <i>tom-1(ok285)</i> neuromuscular synapse prepared by high-pressure freeze and freeze substitution. The presynaptic density is labeled PD, and the plasma membrane-contacting vesicles are indicated with arrows. Scale bar, 100 nm. </p> <p>(B) Vesicles contacting the plasma membrane as a ratio of total vesicles per synaptic profile are increased in <i>tom-1(ok285)</i> mutant animals. </p> <p>(C) The distribution of plasma membrane-contacting vesicles relative to the presynaptic density, expressed in 30-nm bins as a ratio of total vesicles per profile for WT and <i>tom-1(ok285).</i></p> <p>(D) Schematic representation of data in (C) depicting the distribution of vesicles contacting plasma membrane (black circles for WT, purple circles for <i>tom-1</i> mutants) relative to the presynaptic density. Data expressed as mean ± SEM. </p></div>