TY - DATA T1 - Sensitivity of the Total DnaK to the Change in the Transcription Rate Constant for DnaK at Low Temperature PY - 2013/02/22 AU - Hiroyuki Kurata AU - Hana El-Samad AU - Rei Iwasaki AU - Hisao Ohtake AU - John C Doyle AU - Irina Grigorova AU - Carol A Gross AU - Mustafa Khammash UR - https://plos.figshare.com/articles/figure/_Sensitivity_of_the_Total_DnaK_to_the_Change_in_the_Transcription_Rate_Constant_for_DnaK_at_Low_Temperature_/628433 DO - 10.1371/journal.pcbi.0020059.g005 L4 - https://ndownloader.figshare.com/files/958401 KW - dnak KW - transcription N2 - The plot reports the sensitivity computed for the parameters values that satisfy the required yield and efficiency (yield > 0.9 and efficiency > 0.85 at low and high temperature). Yield and efficiency were computed by searching the 2-D parameter space consisting of the transcription rate constant for σ32 and the association constant between σ32 and DnaK as follows: for the various systems, (A) DEG-FB/FF double mutant (SEQ-FB system): K[5] = K[6] = 0 M−1, kx[1] = kx[2] = 0 min−1, η = 1,(B) FF mutant (SEQ-FB + DEG-FB system): K[5] = K[6] = 1 × 108 M−1, kx[1] = kx[2] = 5 min−1, η = 1,(C) Wild-type (SEQ-FB +DEG-FB + FF system): K[5] = K[6] = 1 × 108 M−1, kx[1] = kx[2] = 5 min−1, η = 1 (low), η = 2 (high). ER -