10.1371/journal.pone.0197189.g006 Dongchun Liang Dongchun Liang Jeong-Im Woo Jeong-Im Woo Hui Shao Hui Shao Willi K. Born Willi K. Born Rebecca L. O'Brien Rebecca L. O'Brien Henry J. Kaplan Henry J. Kaplan Deming Sun Deming Sun A2AR<sup>-/-</sup> γδ T cells were unable to suppress Foxp3<sup>+</sup> T cell response. Public Library of Science 2018 γδ T cell cultures Non-activated γδ T cells Foxp 3 T cell interactions adenosine receptors Extracellular adenosine metabolism Activated γδ T cells Foxp 3 T cell expansion γδ T cells γδ T cell-targeted immunotherapies Foxp 3 T cell response 2AR 2018-05-17 08:15:56 Figure https://plos.figshare.com/articles/figure/A2AR_sup_-_-_sup_T_cells_were_unable_to_suppress_Foxp3_sup_sup_T_cell_response_/6283616 <p>A) CD3<sup>+</sup> responder T cells isolated from immunized TCR-δ<sup>-/-</sup> mice were cultured with 1 ng/ml recombinant IL-2 in the presence or absence of γδ T cells isolated from immunized B6 or A2AR<sup>-/-</sup> mice and subsequently stained with a PE-labeled anti-Foxp3 antibody. B) Pretreatment of A2AR<sup>+/+</sup> γδ T cells with an A2AR antagonist (SCH 58261) abolished the enhancing effects. CD3<sup>+</sup> responder T cells isolated from immunized TCR-δ<sup>-/-</sup> mice were cultured with 1 ng/ml recombinant IL-2 for 5 days, in the absence (left panel) or presence (middle and right panels) of γδ T cells that were treated (right panel) or untreated with an A2AR antagonist. Foxp3<sup>+</sup> T cells in the cultures were identified. C) Blockade of high-affinity IL-2 receptor CD25 by monoclonal antibody (PC61) did not affect the inhibiting effect of γδ T cells. CD3<sup>+</sup> responder T cells isolated from immunized TCR-δ<sup>-/-</sup> mice were cultured with 1 ng/ml recombinant IL-2 for 5 days, in the absence (left panel) or presence (three right panels) of activated γδ T cells that were treated as indicated.</p>