TY - DATA T1 - Data_Sheet_1_Characterization of a Pyrethroid-Degrading Pseudomonas fulva Strain P31 and Biochemical Degradation Pathway of D-Phenothrin.PDF PY - 2018/05/16 AU - Jingjing Yang AU - Yanmei Feng AU - Hui Zhan AU - Jie Liu AU - Fang Yang AU - Kaiyang Zhang AU - Lianhui Zhang AU - Shaohua Chen UR - https://frontiersin.figshare.com/articles/dataset/Data_Sheet_1_Characterization_of_a_Pyrethroid-Degrading_Pseudomonas_fulva_Strain_P31_and_Biochemical_Degradation_Pathway_of_D-Phenothrin_PDF/6274145 DO - 10.3389/fmicb.2018.01003.s001 L4 - https://ndownloader.figshare.com/files/11470211 KW - bioremediation KW - D-phenothrin KW - Pseudomonas fulva KW - metabolites KW - degradation pathway N2 - D-phenothrin is one of the most popular pyrethroid insecticides for its broad spectrum and high insecticidal activity. However, continuous use of D-phenothrin has resulted in serious environmental contamination and raised public concern about its impact on human health. Biodegradation of D-phenothrin has never been investigated and its metabolic behaviors remain unknown. Here, a novel bacterial strain P31 was isolated from active sludge, which completely degraded (100%) D-phenothrin at 50 mg⋅L-1 in 72 h. Based on the morphology, 16S rRNA gene and Biolog tests, the strain was identified as Pseudomonas fulva. Biodegradation conditions were optimized as 29.5°C and pH 7.3 by utilizing response surface methodology. Strain P31 depicted high tolerance and strong D-phenothrin degradation ability through hydrolysis pathway. Strain P31 degraded D-phenothrin at inhibition constant (Ki) of 482.1673 mg⋅L-1 and maximum specific degradation constant (qmax) of 0.0455 h-1 whereas critical inhibitor concentration remained as 41.1189 mg⋅L-1. The 3-Phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester were identified as the major intermediate metabolites of D-phenothrin degradation pathway through high-performance liquid chromatography and gas chromatography-mass spectrometry. Bioaugmentation of D-phenothrin-contaminated soils with strain P31 dramatically enhanced its degradation, and over 75% of D-phenothrin was removed from soils within 10 days. Moreover, the strain illustrated a remarkable capacity to degrade other synthetic pyrethroids, including permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin, exhibiting great potential in bioremediation of pyrethroid-contaminated environment. ER -