10.1371/journal.pgen.1007395.g002
August Yue Huang
August Yue
Huang
Xiaoxu Yang
Xiaoxu
Yang
Sheng Wang
Sheng
Wang
Xianing Zheng
Xianing
Zheng
Qixi Wu
Qixi
Wu
Adam Yongxin Ye
Adam
Yongxin Ye
Liping Wei
Liping
Wei
Two types of pSNMs revealed by inter- and intra-organ profiles.
Public Library of Science
2018
Distinctive types
replication timing
late-replicating regions
clonal expansion events
mutation rate
CpG sites
clonal expansion pSNMs
whole-genome sequencing
postzygotic mosaicism
27 postmortem organ samples
allele fractions
organs Postzygotic single-nucleotide mosaicisms
chromatin states
ultra-deep amplicon re-sequencing
intra-organ comparison
postzygotic single-nucleotide mosaicisms
164 pSNMs
2018-05-15 17:37:24
Figure
https://plos.figshare.com/articles/figure/Two_types_of_pSNMs_revealed_by_inter-_and_intra-organ_profiles_/6272015
<p>(A) Minor allele fractions between different categories of pSNM. The organ-shared pSNMs demonstrated significantly higher allele fractions than the organ-unique pSNMs. (B) Number of pSNMs carried in different organ samples. Red and blue bars denote the organ-shared and organ-unique pSNMs, respectively. An excess of organ-unique pSNMs was observed in the breast sample of BBL11121 and the liver sample of BBLD1005. (C-D) Heatmap of minor allele fractions for pSNMs carried in multiple organ samples of BBLD1005 (C) and BBL11121 (D). Blood samples of two unrelated individuals (ACC1 and ACC4) served as negative controls. The color intensity of each tile represents allele fractions estimated by targeted ultra-depth resequencing. Gray tiles denote sites without sufficient read depth (< 30X). Red and blue bars denote the organ-shared and organ-unique pSNMs, respectively. The majority of organ-unique pSNMs were locally restricted to one or a few physically adjacent organ samples (<1 cm). (E-F) Relative intra-organ similarity of pSNM profiles in BBLD1005 (E) and BBL11121 (F). The originally sequenced samples (liver #9 and breast #9) shared the largest similarity to their physically closest samples.</p>