%0 Generic %A Edskes, Herman K. %A Mukhamedova, Maryam %A Edskes, Bouke K. %A Wickner, Reed B. %D 2018 %T Supplemental Material for Edskes et al., 2018 %U https://gsajournals.figshare.com/articles/dataset/Supplemental_Material_for_Edskes_et_al_2018/6205691 %R 10.25386/genetics.6205691.v1 %2 https://ndownloader.figshare.com/files/11283053 %2 https://ndownloader.figshare.com/files/11283044 %2 https://ndownloader.figshare.com/files/11283047 %2 https://ndownloader.figshare.com/files/11283041 %2 https://ndownloader.figshare.com/files/11283056 %2 https://ndownloader.figshare.com/files/11283050 %K LUG1/YLR352W %K F-box protein %K Ure2p %K nitrogen catabolite repression %K Hap4p %K prion %K URE3 %K gln1 %K Genetics %X Supplemental Methods_figures_tables.pdf - Provides a detailed description of the culture conditions, induction of Hermes transposition, selection of colonies carrying a transposition, extraction of cellular DNA, PCR amplification and isolation of the junction points between transposon and chromosomal insertion site, next-generation sequencing of these sites, and analysis of the data by visual display and by counting insertions per open reading frame.

Exon Intron Counts.xlsx - Gives the insertions in every yeast open reading frame, distinguishing exons from introns where appropriate.

Sorted Hits.xlsx - Gives prominent hits sorted by functional group, “TY gag-pol Counts.xlsx comparing insertions in the Ty retrotransposons at different locations in the genome.

Count Insertions in ORFs and Introns.txt - The Python program used for counting insertions.

LUGsIGV-InsertDistributions.pptx - Is a slide show of insert distributions in each of 500 genes for which insertions were recovered more frequently in [ure-o] than in [URE3] cultures. %I GSA Journals