10.1371/journal.pgen.0030108.g004 Joyce van de Leemput Joyce van de Leemput Jayanth Chandran Jayanth Chandran Melanie A Knight Melanie A Knight Lynne A Holtzclaw Lynne A Holtzclaw Sonja Scholz Sonja Scholz Mark R Cookson Mark R Cookson Henry Houlden Henry Houlden Katrina Gwinn-Hardy Katrina Gwinn-Hardy Hon-Chung Fung Hon-Chung Fung Xian Lin Xian Lin Dena Hernandez Dena Hernandez Javier Simon-Sanchez Javier Simon-Sanchez Nick W Wood Nick W Wood Paola Giunti Paola Giunti Ian Rafferty Ian Rafferty John Hardy John Hardy Elsdon Storey Elsdon Storey R. J. McKinlay Gardner R. J. McKinlay Gardner Susan M Forrest Susan M Forrest Elizabeth M. C Fisher Elizabeth M. C Fisher James T Russell James T Russell Huaibin Cai Huaibin Cai Andrew B Singleton Andrew B Singleton Western Blot Analysis of ITPR1 Protein Levels in EBV Immortalized Lymphoblasts from AUS1 Family Members Public Library of Science 2007 blot itpr1 ebv immortalized lymphoblasts aus1 2007-06-22 02:37:16 Figure https://plos.figshare.com/articles/figure/_Western_Blot_Analysis_of_ITPR1_Protein_Levels_in_EBV_Immortalized_Lymphoblasts_from_AUS1_Family_Members_/619436 <p>Western blot performed to examine ITPR1 levels in EBV immortalized lymphocytes from AUS1 affected family members carrying the <i>ITPR1</i> deletion and from an AUS1 family member of unknown disease status who does not carry the deletion. Notably the samples from patients with <i>ITPR1</i> deletion show a dramatic decrease in ITPR1 levels. To demonstrate equal loading, these samples were diluted one in five, and the Western blot was repeated using an antibody against ACTB.</p>