%0 Figure %A T. Nitta, Ryan %A L. Smith, Catherine %A K. Kennedy, Brian %D 2007 %T Overexpression of gankyrin reduces pRB levels and makes Lmna+/+ cells refractory to p16ink4a-induced arrest. %U https://plos.figshare.com/articles/figure/_Overexpression_of_gankyrin_reduces_pRB_levels_and_makes_Lmna_cells_refractory_to_p16_ink4a_induced_arrest_/615058 %R 10.1371/journal.pone.0000963.g003 %2 https://ndownloader.figshare.com/files/944675 %K gankyrin %K prb %K makes %K cells %K refractory %X

(A) Western analysis of Lmna+/+ infected with a control plasmid, Lmna+/+-Cont, or gankyrin, Lmna+/+-Gank, and uninfected Lmna−/− fibroblasts. Rb−/− fibroblasts were included as a control for pRB levels. (B) Cells were transfected with GFP-p16ink4a or GFP-p21cip1 and GFP-positive cells were scored; S-phase cells were identified by BrdU incorporation. The percentage of cells positive for GFP and BrdU incorporation were quantified. Results are shown with standard deviations from triplicate samples; 150 cells were counted per sample. (C) Flow cytometry was conducted on Lmna+/+-Cont and Lmna+/+-Gank cells transfected with GFP or GFP-p16ink4a.

%I PLOS ONE