Functional and transcriptional differences between degranulating cells in LN and blood. Marcus Buggert Son Nguyen Laura M. McLane Maria Steblyanko Nadia Anikeeva Dominic Paquin-Proulx Perla M. Del Rio Estrada Yuria Ablanedo-Terrazas Kajsa Noyan Morgan A. Reuter Korey Demers Johan K. Sandberg Michael A. Eller Hendrik Streeck Marianne Jansson Piotr Nowak Anders Sönnerborg David H. Canaday Ali Naji E. John Wherry Merlin L. Robb Steven G. Deeks Gustavo Reyes-Teran Yuri Sykulev Annika C. Karlsson Michael R. Betts 10.1371/journal.ppat.1006973.g006 https://plos.figshare.com/articles/figure/Functional_and_transcriptional_differences_between_degranulating_cells_in_LN_and_blood_/6141356 <p><b>(A)</b> Flow cytometry plots (HIV-uninfected subject) of matched LN and PB CD107a+ SEB stimulated CD4+ T cell responses. Graphs are showing the frequencies of LN (top) and PB (bottom) CD107a+ SEB stimulated CD4+ T cell responses for CXCR5-, CXCR5+ and CXCR5<sup>hi</sup> cells. <b>(B)</b> Flow plots (HIV-infected CP) illustrating the expression of CD107a+ (red) Gag-specific CD4+ T cells in relation to CXCR5 between LN (top) and PB (bottom). <b>(C)</b> Corresponding plots from the same subject showing the expression of CD107a+ (red) Gag-specific CD4+ T cells in relation to CXCR5 and perforin for LN (top) and PB (bottom). Graphs represent the frequency of CD107a+ cells within the CXCR5+, CXCR5<sup>hi</sup> and perforin+ compartment for LN (top) and PB (bottom) Gag-specific CD4+ T cells. <b>(D)</b> Biomark analysis illustrating the tSNE distribution of single SEB stimulated CD107+ CD4+ T cells from LN (black) and PB (gray). Individual graphs represent the relative Log2 expression of different markers being significantly different (P<0.05) between blood and LN CD107+ cells. Non-parametric Kruskal Wallis test with Dunn’s multiple comparison test was performed to determine significant differences between groups; *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001. All data-points are derived from the North-American and Mexico cohort.</p> 2018-04-13 17:45:21 HIV replication T-box transcription factors T-bet transcriptional effector T cell properties lack effector functions IFN cytolytic molecules HIV elite controllers LN lymphoid tissues chemokine