Functional and transcriptional differences between degranulating cells in LN and blood.
Marcus Buggert
Son Nguyen
Laura M. McLane
Maria Steblyanko
Nadia Anikeeva
Dominic Paquin-Proulx
Perla M. Del Rio Estrada
Yuria Ablanedo-Terrazas
Kajsa Noyan
Morgan A. Reuter
Korey Demers
Johan K. Sandberg
Michael A. Eller
Hendrik Streeck
Marianne Jansson
Piotr Nowak
Anders Sönnerborg
David H. Canaday
Ali Naji
E. John Wherry
Merlin L. Robb
Steven G. Deeks
Gustavo Reyes-Teran
Yuri Sykulev
Annika C. Karlsson
Michael R. Betts
10.1371/journal.ppat.1006973.g006
https://plos.figshare.com/articles/figure/Functional_and_transcriptional_differences_between_degranulating_cells_in_LN_and_blood_/6141356
<p><b>(A)</b> Flow cytometry plots (HIV-uninfected subject) of matched LN and PB CD107a+ SEB stimulated CD4+ T cell responses. Graphs are showing the frequencies of LN (top) and PB (bottom) CD107a+ SEB stimulated CD4+ T cell responses for CXCR5-, CXCR5+ and CXCR5<sup>hi</sup> cells. <b>(B)</b> Flow plots (HIV-infected CP) illustrating the expression of CD107a+ (red) Gag-specific CD4+ T cells in relation to CXCR5 between LN (top) and PB (bottom). <b>(C)</b> Corresponding plots from the same subject showing the expression of CD107a+ (red) Gag-specific CD4+ T cells in relation to CXCR5 and perforin for LN (top) and PB (bottom). Graphs represent the frequency of CD107a+ cells within the CXCR5+, CXCR5<sup>hi</sup> and perforin+ compartment for LN (top) and PB (bottom) Gag-specific CD4+ T cells. <b>(D)</b> Biomark analysis illustrating the tSNE distribution of single SEB stimulated CD107+ CD4+ T cells from LN (black) and PB (gray). Individual graphs represent the relative Log2 expression of different markers being significantly different (P<0.05) between blood and LN CD107+ cells. Non-parametric Kruskal Wallis test with Dunn’s multiple comparison test was performed to determine significant differences between groups; *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001. All data-points are derived from the North-American and Mexico cohort.</p>
2018-04-13 17:45:21
HIV replication
T-box transcription factors T-bet
transcriptional effector T cell properties
lack effector functions
IFN
cytolytic molecules
HIV elite controllers
LN
lymphoid tissues
chemokine