Functional characteristics of polyclonal and virus-specific effector CD4+ T cell responses in HIV-infected LNs and blood. Marcus Buggert Son Nguyen Laura M. McLane Maria Steblyanko Nadia Anikeeva Dominic Paquin-Proulx Perla M. Del Rio Estrada Yuria Ablanedo-Terrazas Kajsa Noyan Morgan A. Reuter Korey Demers Johan K. Sandberg Michael A. Eller Hendrik Streeck Marianne Jansson Piotr Nowak Anders Sönnerborg David H. Canaday Ali Naji E. John Wherry Merlin L. Robb Steven G. Deeks Gustavo Reyes-Teran Yuri Sykulev Annika C. Karlsson Michael R. Betts 10.1371/journal.ppat.1006973.g004 https://plos.figshare.com/articles/figure/Functional_characteristics_of_polyclonal_and_virus-specific_effector_CD4_T_cell_responses_in_HIV-infected_LNs_and_blood_/6141344 <p><b>(A)</b> Flow cytometry plots (HIV ART+ subject) and plots for matched HIV-Gag or–Env-specific CD4+ T cell responses in HIV-infected LN and PB. <b>(B)</b> Flow cytometry plots (HIV-infected CP) showing the negative control (NC) and Gag-specific response of LN CD4+ T cell response. The high abundance of CD107a (red) that is not co-expressed with IFNγ or TNF is illustrated in this example. SPICE analysis of functional combination between LN (red) and PB (red-gray) Gag-specific CD4+ T cell responses for HIV-infected CPs and ART+ subjects. <b>(C)</b> Flow plots (HIV-infected CP) of Gag-specific CD4+ T cell response (red) from LN and PB in relation to CD27 and perforin expression. Graphs represent the frequency of <b>(C)</b> perforin+ and <b>(D)</b> T-bet<sup>hi</sup> cells between LN and PB Gag-specific CD4+ T cells. <b>(E)</b> Flow plots (HIV-infected CP) of MIP-1α <i>versus</i> IFNγ and TNF production for LN and PB SEB stimulated CD4+ T cells. Corresponding plots showing the frequency of MIP-1α+ SEB stimulated CD4+ T cells (top) and MIP-1α+ of IFNγ/TNF/CD107a/MIP-1α+ SEB stimulated CD4+ T cells (bottom). <b>(F)</b> Flow plots (HIV-infected CP) of MIP-1α <i>versus</i> IFNγ and TNF production for LN and PB CMV-specific CD4+ T cells and corresponding graphs showing the frequency of MIP-1α+ CMV-specific CD4+ T cells (top) and MIP-1α+ of IFNγ/TNF/CD107a/MIP-1α+ CMV-specific CD4+ T cells (bottom). Median and IQR are shown for all bar plots. Permutation test was performed between the pie charts. Wilcoxon matched-pairs single rank tests were performed to compare differences between two matched groups; *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001. All data-points are derived from the Mexico cohort.</p> 2018-04-13 17:45:21 HIV replication T-box transcription factors T-bet transcriptional effector T cell properties lack effector functions IFN cytolytic molecules HIV elite controllers LN lymphoid tissues chemokine