10.1371/journal.ppat.1006973.g003
Marcus Buggert
Marcus
Buggert
Son Nguyen
Son
Nguyen
Laura M. McLane
Laura M.
McLane
Maria Steblyanko
Maria
Steblyanko
Nadia Anikeeva
Nadia
Anikeeva
Dominic Paquin-Proulx
Dominic
Paquin-Proulx
Perla M. Del Rio Estrada
Perla M.
Del Rio Estrada
Yuria Ablanedo-Terrazas
Yuria
Ablanedo-Terrazas
Kajsa Noyan
Kajsa
Noyan
Morgan A. Reuter
Morgan
A. Reuter
Korey Demers
Korey
Demers
Johan K. Sandberg
Johan
K. Sandberg
Michael A. Eller
Michael
A. Eller
Hendrik Streeck
Hendrik
Streeck
Marianne Jansson
Marianne
Jansson
Piotr Nowak
Piotr
Nowak
Anders Sönnerborg
Anders
Sönnerborg
David H. Canaday
David
H. Canaday
Ali Naji
Ali
Naji
E. John Wherry
E.
John Wherry
Merlin L. Robb
Merlin
L. Robb
Steven G. Deeks
Steven
G. Deeks
Gustavo Reyes-Teran
Gustavo
Reyes-Teran
Yuri Sykulev
Yuri
Sykulev
Annika C. Karlsson
Annika
C. Karlsson
Michael R. Betts
Michael
R. Betts
Phenotypic, cytolytic and transcriptional differences between LN and blood CD4+ T cells in HIV-infected and -uninfected individuals.
Public Library of Science
2018
HIV replication
T-box transcription factors T-bet
transcriptional effector T cell properties
lack effector functions
IFN
cytolytic molecules
HIV elite controllers
LN
lymphoid tissues
chemokine
2018-04-13 17:45:21
Figure
https://plos.figshare.com/articles/figure/Phenotypic_cytolytic_and_transcriptional_differences_between_LN_and_blood_CD4_T_cells_in_HIV-infected_and_-uninfected_individuals_/6141341
<p><b>(A)</b> Flow cytometry plots (HIV-infected CP) and scatter plots for naïve and memory subsets of LN and peripheral blood (PB) CD4+ T cells in HIV-infected and -uninfected subjects. <b>(B)</b> Flow cytometry plots (HIV-infected CP) showing the lack of T-bet<sup>hi</sup>Eomes+ CD4+ T cells in LNs. Corresponding scatter plots demonstrating the frequency of T-bet<sup>hi</sup> cells of memory (non-naïve) CD4+ T cells (top) and frequency of Eomes+ cells of T-bet<sup>hi</sup> CD4+ T cells (bottom) for matched LN and PB. <b>(C)</b> Flow plots (HIV-infected CP) showing the lack of Granzyme B+perforin+ CD4+ T cells in LNs and scatter plots with the frequency of LN and PB perforin+ cells of memory CD4+ T cells (top). Frequencies of Granzyme B+ cells of perforin+ CD4+ T cells (bottom) for matched LN and PB. <b>(D)</b> Flow plots (HIV-infected CP) and scatter plots showing the distribution of CD27+ cells within the Granzyme B+ CD4+ T cell compartment for matched LN and PB. <b>(E)</b> The distribution of different populations in the tSNE space is based on 30.000 live CD4+ T cells that were merged from LN and PB from a HIV-infected CP with detectable levels of cytolytic cells in the PB and LN Tfh cells. The tSNE clustering is based on CD45RO, CD27, CCR7, T-bet, Eomes, Granzyme B, perforin, CXCR5 and PD-1 expression on gated bulk CD4+ T cells. The naïve cluster (green) is based on high CCR7 and low CD45RO intensity; the Tfh cluster (red) on high intensity of PD-1 and CXCR5; and the effector cluster (orange) on high T-bet and perforin expression intensity. After separating out the merged LN and PB single CD4+ T cell data, a lack of Tfh cells was apparent in PB and effector CD4+ T cells in the LN (lower right tSNE plots). Median and IQR are shown for all scatter plots. Mann-Whitney tests were performed to compare differences between two unmatched groups, and Wilcoxon matched-pairs single rank tests between matched samples; *<i>P</i> < 0.05, **<i>P</i> < 0.01 and ***<i>P</i> < 0.001. All data-points are derived from the North-American and Mexico cohort.</p>