10.4225/03/5ad3e663412f8
HON WEI NG
WEI
NG
Polymer modified ion exchange adsorbent for the flow-through chromatography of hepatitis B virus-like particle
Monash University
2018
flow-through chromatography
hepatitis B virus-like particles
anion exchange chromatography
poly(oligo(ethylene glycol) methacrylate)
size-selective adsorption
Bioprocessing, Bioproduction and Bioproducts
2018-04-15 23:55:13
Thesis
https://bridges.monash.edu/articles/thesis/Polymer_modified_ion_exchange_adsorbent_for_the_flow-through_chromatography_of_hepatitis_B_virus-like_particle/6127829
Size selective flow-through chromatography (FC) was employed to adsorb impurities i.e. host cell proteins while large hepatitis B core antigen virus like particles (HB-VLPs) are recovered in the flow-through fraction. An inert polymer was grafted on adsorbents to improve the performance. The effect of feedstock conditions (size of target protein and protein load) and adsorbent design (adsorbent size, type of polymer chain and POEGMA architecture) on the performance of FC were investigated. Large size of HB-VLPs limits the protein load to 7 mg/ml, while optimal adsorbent design improved flow-through recovery by 5% compared to previous studies.