TY - DATA T1 - Identification of NEM-Modified Cysteines in ALV-A Env by Mass Spectrometry PY - 2013/02/21 AU - Jason G Smith AU - James M Cunningham UR - https://plos.figshare.com/articles/figure/_Identification_of_NEM_Modified_Cysteines_in_ALV_A_Env_by_Mass_Spectrometry_/611385 DO - 10.1371/journal.ppat.0030198.g002 L4 - https://ndownloader.figshare.com/files/940958 KW - nem-modified KW - cysteines KW - alv-a KW - env N2 - (A) ALV-A was exposed to PMB or to NHS-Biotin in the presence or absence of sTva. Env was immunoprecipitated with a polyclonal antibody against the C-terminus of TM and biotinylated proteins were detected with streptavidin-HRP. The positions of SU and TM are indicated.(B) The purified, deglycosylated SU used for mass spectrometry analysis was quantified by SYPRO Ruby staining. The positions of SU and the PNGase F enzyme used for glycosylation are indicated. Molecular weight standards are in the left lane.(C) Mass spectrometry identified three peptides from SU containing NEM-modified cysteine residues (indicated by *).(D) Vertical bars represent the cysteines in SU (two of the cysteines are sequential just before position 292) and are positioned to scale. The host range (HR) determinants 1 and 2 are indicated in light grey for reference. The NEM-modified cysteines are numbered. ER -