RNA Conformational Changes That Differentiate Ex Virio and In Vitro Transcript States
Kevin A Wilkinson
Robert J Gorelick
Suzy M Vasa
Nicolas Guex
Alan Rein
David H Mathews
Morgan C Giddings
Kevin M Weeks
10.1371/journal.pbio.0060096.g005
https://plos.figshare.com/articles/figure/_RNA_Conformational_Changes_That_Differentiate_Ex_Virio_and_In_Vitro_Transcript_States_/603518
<div><p>(A) The in vitro transcript forms a loop-loop dimer. Identical samples of the transcript RNA were resolved in nondenaturing gels, either omitting or containing 7 mM MgCl<sub>2</sub>. Markers for monomeric (M) and dimeric (D) conformations are shown.</p>
<p>(B) SHAPE reactivity as a function of nucleotide position for ex virio (red) and transcript (black) RNAs. Difference plot in lower panel has been smoothed over a 5-nt window.</p>
<p>(C) Secondary structure model for the primer binding site in the absence of tRNA(lys3).</p></div>
2008-04-29 00:58:38
conformational
differentiate
ex
virio
vitro
transcript