TY - DATA T1 - Combined inactivation of MYC and K-rasG12D in lung tumor cells results in a shutdown of Stat3 signaling. PY - 2008/05/07 AU - Phuoc T. Tran AU - Alice C. Fan AU - Pavan K. Bendapudi AU - Shan Koh AU - Kim Komatsubara AU - Joy Chen AU - George Horng AU - David I. Bellovin AU - Sylvie Giuriato AU - Craig S. Wang AU - Jeffrey A. Whitsett AU - Dean W. Felsher UR - https://plos.figshare.com/articles/figure/_Combined_inactivation_of_MYC_and_K_ras_G12D_in_lung_tumor_cells_results_in_a_shutdown_of_Stat3_signaling_/603506 DO - 10.1371/journal.pone.0002125.g007 L4 - https://ndownloader.figshare.com/files/933082 KW - inactivation KW - myc KW - cells KW - shutdown KW - stat3 N2 - (A) Representative phospho-Stat5 and (B) phospho-Stat3 IHC analysis demonstrates little to no levels of nuclear staining in cells following dual inactivation of MYC/K-rasG12D (n = 3 “On” & 6 “Off”) similar to conditional K-rasG12D–induced tumors. MYC/K-ras #2-3 represent independent inactivated tumors with no to highest amount of staining observed, respectively. IHC was performed similar to Figure 1D with stated antibodies with CMR-induced lung tumors that were activated or inactivated (2–11 weeks). Adjacent bar graph panels represent scoring of individual tumors for IHC staining: negative, low (<50% positive cells) or high (≥50% positive cells) for phospho-Stat5 and phospho-Stat3 positive tumors. ER -