10.1371/journal.pone.0002125.g007 Phuoc T. Tran Phuoc T. Tran Alice C. Fan Alice C. Fan Pavan K. Bendapudi Pavan K. Bendapudi Shan Koh Shan Koh Kim Komatsubara Kim Komatsubara Joy Chen Joy Chen George Horng George Horng David I. Bellovin David I. Bellovin Sylvie Giuriato Sylvie Giuriato Craig S. Wang Craig S. Wang Jeffrey A. Whitsett Jeffrey A. Whitsett Dean W. Felsher Dean W. Felsher Combined inactivation of MYC and <i>K-ras<sup>G12D</sup></i> in lung tumor cells results in a shutdown of Stat3 signaling. Public Library of Science 2008 inactivation myc cells shutdown stat3 2008-05-07 00:58:26 Figure https://plos.figshare.com/articles/figure/_Combined_inactivation_of_MYC_and_K_ras_G12D_in_lung_tumor_cells_results_in_a_shutdown_of_Stat3_signaling_/603506 <p>(A) Representative phospho-Stat5 and (B) phospho-Stat3 IHC analysis demonstrates little to no levels of nuclear staining in cells following dual inactivation of MYC/<i>K-ras<sup>G12D</sup></i> (n = 3 “On” & 6 “Off”) similar to conditional <i>K-ras<sup>G12D</sup></i>–induced tumors. MYC/K-ras #2-3 represent independent inactivated tumors with no to highest amount of staining observed, respectively. IHC was performed similar to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002125#pone-0002125-g001" target="_blank">Figure 1D</a> with stated antibodies with CMR-induced lung tumors that were activated or inactivated (2–11 weeks). Adjacent bar graph panels represent scoring of individual tumors for IHC staining: negative, low (<50% positive cells) or high (≥50% positive cells) for phospho-Stat5 and phospho-Stat3 positive tumors.</p>