Habjan, Matthias Andersson, Ida Klingström, Jonas Schümann, Michael Martin, Arnold Zimmermann, Petra Wagner, Valentina Pichlmair, Andreas Schneider, Urs Mühlberger, Elke Mirazimi, Ali Weber, Friedemann Genomic RNAs of segmented NSVs. <p>RNAs isolated from virus particles of LASV (A) and RVFV (B) were tested for their ability to activate the IFN-β promoter in dependency of RIG-I or MDA5 (left panels) or 5′ triphosphate groups (right panels). (C) IFN-β promoter activation by genomic RNAs of the bunyaviruses RVFV, HTNV, and CCHFV. Mean values and standard deviations from 3 independent experiments are shown. (D) Purified virion RNAs (5 µg) of RVFV, HTNV, and CCHFV were separated on denaturing formaldehyde agarose gels. The genome segments (L, large; M, middle; S, small) are labeled on the right, and asterisks indicate 28S and 18S rRNA bands. Contamination of virus preparations with ribosomal RNAs are often observed <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002032#pone.0002032-Staunton1" target="_blank">[49]</a> but have no consequences for IFN induction. As a control RNA isolated from ultracentrifuged supernatants of uninfected cells is shown (mock).</p> rnas;segmented 2013-02-21
    https://plos.figshare.com/articles/figure/_Genomic_RNAs_of_segmented_NSVs_/602693
10.1371/journal.pone.0002032.g003