%0 Figure %A Girinathan, Brintha Parasumanna %A Ou, Junjun %A Dupuy, Bruno %A Govind, Revathi %D 2018 %T Mutation in the sin locus affects C. difficile flagellar synthesis. %U https://plos.figshare.com/articles/figure/Mutation_in_the_i_sin_i_locus_affects_i_C_i_i_difficile_i_flagellar_synthesis_/5974951 %R 10.1371/journal.ppat.1006940.g004 %2 https://ndownloader.figshare.com/files/10830086 %K SinR %K JIR %K sporulation %K strains R 20291 %K sin locus mutants %K 0A %K sin locus %K sin locus Clostridium %K target gene expression %K aa %K toxin production %X

(A) Heat map showing the lower expression of flagellar and motility-related genes in the R2091::sinRR’ mutant compared to the parent. Color intensity in each cell represents corresponding Log2 expression values in the color scale bar. (B) Transmission electron micrographs of negatively stained C. difficile cells. White arrows point to flagella. (C) Motility of R20291, sinRR’ mutant and the sinRR’ mutant complemented strains in BHIS with 0.3% agar. The sigD mutant and the sinRR’ mutant expressing sigD from an inducible promoter were included in this analysis. The swim diameters (mm) was measured every 24 h for a total of 120 h is shown and the data shown are means ± standard errors of three biological replicates. The experiments were repeated at least three times independently (*, p≤0.05 by a two-tailed Student's t-test).

%I PLOS Pathogens