TY - DATA T1 - Transcriptomic analysis of Arabidopsis thaliana plants treated with the Ky-9 and Ky-72 histone deacetylase inhibitors PY - 2018/03/08 AU - Huong Mai Nguyen AU - Kaori Sako AU - Akihiro Matsui AU - Minoru Ueda AU - Maho Tanaka AU - Akihiro Ito AU - Norikazu Nishino AU - Minoru Yoshida AU - Motoaki Seki UR - https://tandf.figshare.com/articles/dataset/Transcriptomic_analysis_of_i_Arabidopsis_thaliana_i_plants_treated_with_the_Ky-9_and_Ky-72_histone_deacetylase_inhibitors/5965165 DO - 10.6084/m9.figshare.5965165.v1 L4 - https://ndownloader.figshare.com/files/10689274 L4 - https://ndownloader.figshare.com/files/10689277 L4 - https://ndownloader.figshare.com/files/10689280 L4 - https://ndownloader.figshare.com/files/10689283 L4 - https://ndownloader.figshare.com/files/10689286 KW - Epigenetics KW - high-salinity stress KW - histone deacetylase inhibitor KW - histone acetylation KW - transcriptome N2 - Histone acetylation plays a pivotal role in plant growth and development, and is regulated by the antagonistic relationship between histone acetyltransferase (HAT) and histone deacetylase (HDAC). We previously revealed that some HDAC inhibitors confer high-salinity stress tolerance in plants. In this study, we identified two HDAC inhibitors, namely Ky-9 and Ky-72, which enhanced the high-salinity stress tolerance of Arabidopsis thaliana. Ky-9 and Ky-72 are structurally similar chlamydocin analogs. However, the in vitro inhibitory activity of Ky-9 against mammalian HDAC is greater than that of Ky-72. A western blot indicated that Ky-9 and Ky-72 increased the acetylation levels of histone H3, suggesting they exhibit HDAC inhibitory activities in plants. We conducted a transcriptomic analysis to investigate how Ky-9 and Ky-72 enhance high-salinity stress tolerance. Although Ky-9 upregulated the expression of more genes than Ky-72, similar gene expression patterns were induced by both HDAC inhibitors. Additionally, the expression of high-salinity stress tolerance-related genes, such as anthocyanin-related genes and a small peptide-encoding gene, increased by Ky-9 and Ky-72. These data suggest that slight structural differences in chemical side chain between HDAC inhibitors can alter inhibitory effect on HDAC protein leading to influence gene expression, thereby enhancing high-salinity stress tolerance in different extent. ER -