10.1371/journal.ppat.1006948.g002
Zhulong Hu
Zhulong
Hu
Yuang Wang
Yuang
Wang
Qiaopeng Tang
Qiaopeng
Tang
Xiaodan Yang
Xiaodan
Yang
Yali Qin
Yali
Qin
Mingzhou Chen
Mingzhou
Chen
CHX treatment induces HPIV3-triggered SG disassembly.
Public Library of Science
2018
parainfluenza virus type 3
N L 478A
eIF 2α phosphorylation
RNAs Viral invasion triggers
HPIV
replication
IB
response
stress granule formation
SG formation
2018-03-08 18:50:32
Figure
https://plos.figshare.com/articles/figure/CHX_treatment_induces_HPIV3-triggered_SG_disassembly_/5965045
<p>(A and B) HeLa cells were treated with AS (0.5 mM) for 30 min and subsequently treated with or without CHX in the presence of AS for another 1 h. (A) Cells were immunostained for TIA-1 (green) and G3BP (red). Nuclei were stained with DAPI (blue). The white scale bar corresponds to 10 μm. (B) The percentage of cells containing SGs was quantified in three independent experiments. (C-H) HeLa cells were mock-infected or infected with HPIV3 (MOI = 1) for 24 h and subsequently mock-treated or treated with CHX for another 1 h, 2 h, or 3 h. (C) Cells were immnostained for HPIV3 (purple), TIA-1 (green), and G3BP (red). Nuclei were stained with DAPI (blue). The white scale bar corresponds to 10 μm. (D) The percentage of cells containing SGs was quantified in three independent experiments. (E) Cell lysates were analyzed via western blot using anti-HN and anti-GAPDH antibodies. (F) Western blots from three independent experiments were quantified and normalized with respect to the amount of GAPDH. (G and H) Total RNA were isolated for qPCR analysis to measure the indicated RNA abundance and normalized to that of GAPDH. Data are represented as means ±SD. Student’s t test: * P<0.05, ** P<0.01, *** P<0.001, ns = not significant.</p>