Makidon, Paul E. Bielinska, Anna U. S. Nigavekar, Shraddha W. Janczak, Katarzyna Knowlton, Jessica J. Scott, Alison Mank, Nicholas Cao, Zhengyi Rathinavelu, Sivaprakash R. Beer, Michael Erby Wilkinson, J. P. Blanco, Luz J. Landers, Jeffrey R. Baker Jr, James Development of IgG response in serum. <p>Effect of NE adjuvant: Mice were immunized intranasally with HBsAg-NE consisting of 20 μg of HBsAg mixed with 0% to 40% concentrations of NE (A). Significant statistical difference with <i>p value<0.05</i> was observed between all HBsAg-NE formulations and HBsAg-PBS vaccine. Antigen dose escalation: Mice were immunized intranasally with 1 μg to 40 μg HBsAg mixed with 20% NE (B). A significant statistical difference with <i>p value<0.05</i> was observed between all 5 μg to 40 μg HBsAg-NE formulations and 1 μg HBsAg-NE vaccine. Serum anti-HBsAg IgG antibody concentrations are presented as mean of endpoint titers in individual sera +/− SEM. * indicates a statistical difference (<i>p value<0.05</i>) in the anti-HBsAg IgG titers. Arrows indicate vaccine administration.</p> igg 2013-02-21
    https://plos.figshare.com/articles/figure/_Development_of_IgG_response_in_serum_/594684
10.1371/journal.pone.0002954.g003