10.1371/journal.pone.0002954.g003 Paul E. Makidon Paul E. Makidon Anna U. Bielinska Anna U. Bielinska Shraddha S. Nigavekar Shraddha S. Nigavekar Katarzyna W. Janczak Katarzyna W. Janczak Jessica Knowlton Jessica Knowlton Alison J. Scott Alison J. Scott Nicholas Mank Nicholas Mank Zhengyi Cao Zhengyi Cao Sivaprakash Rathinavelu Sivaprakash Rathinavelu Michael R. Beer Michael R. Beer J. Erby Wilkinson J. Erby Wilkinson Luz P. Blanco Luz P. Blanco Jeffrey J. Landers Jeffrey J. Landers James R. Baker Jr James R. Baker Jr Development of IgG response in serum. Public Library of Science 2013 igg 2013-02-21 09:46:20 Figure https://plos.figshare.com/articles/figure/_Development_of_IgG_response_in_serum_/594684 <p>Effect of NE adjuvant: Mice were immunized intranasally with HBsAg-NE consisting of 20 μg of HBsAg mixed with 0% to 40% concentrations of NE (A). Significant statistical difference with <i>p value<0.05</i> was observed between all HBsAg-NE formulations and HBsAg-PBS vaccine. Antigen dose escalation: Mice were immunized intranasally with 1 μg to 40 μg HBsAg mixed with 20% NE (B). A significant statistical difference with <i>p value<0.05</i> was observed between all 5 μg to 40 μg HBsAg-NE formulations and 1 μg HBsAg-NE vaccine. Serum anti-HBsAg IgG antibody concentrations are presented as mean of endpoint titers in individual sera +/− SEM. * indicates a statistical difference (<i>p value<0.05</i>) in the anti-HBsAg IgG titers. Arrows indicate vaccine administration.</p>