%0 Generic %A Dworkin, Ian %A Sivaratnam, Gayatri %D 2018 %T Images from Chandler, Chari et al. 2017 PLoS Genetics "How well do you know your mutation? Complex effects of genetic background on expressivity, complementation, and ordering of allelic effects" %U https://figshare.com/articles/dataset/Images_from_Chandler_Chari_et_al_2017_PLoS_Genetics_How_well_do_you_know_your_mutation_Complex_effects_of_genetic_background_on_expressivity_complementation_and_ordering_of_allelic_effects_/5944852 %R 10.6084/m9.figshare.5944852.v1 %2 https://ndownloader.figshare.com/files/10637236 %K Drosophila melanogaster %K Genetic Background Effects %K images %K morphology %K Drosophila wings %K scalloped %K Genetics %K Developmental Genetics (incl. Sex Determination) %X These are the images associated with the data collected for

http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007075

For the zipped folder GS_data:
Data Provenance (how data was collected):
via dissections, area imaging
Author:
Performed - Ian Dworkin, Gayatri Sivaratnam, Samiksha Kaul
Dissections - Gayatri Sivaratnam, Samiksha Kaul
Imaging - I. Dworkin, G. Sivaratnam, Samiksha Kaul

Dates
samples Collected: July 21, 2015
Dissection: August 17 - September25, 2015
Imging: September 30, 2015 (ID, GS, SK)
(Date samples were collected, dates for imaging)

A bit about the experiment:
16 DGRP lines were crossed to 3 mutations (sd 1, sd E3, sd 58d) and a wildtype (SAM)
The effects of intermediate mutation on different lines, whether the severity of this effect varied across the different lines is being investigated.

Microscope, camera used. Leica m125 with a Leica DFC400 camera, using Leica Application Software 3.4.0. Greyscale images.

Each image is 1392 x 1040 px. Real size 2.59 x 1.93 mm

Magnification : 50X (0.5X?)
aperture about halfway across (59%)
Brightness: 68%
Gamma: 0.80

Notes:
naming convention for images: SAM/sd1/sde3/sd58d_line#_M_1/2/3/4/5/6.TIF

SAM/sd1/sde3/sd58d - either the wild type (SAM) allele for scalloped, or allele 1, E3 or 58d %I figshare