%0 Generic
%A Dworkin, Ian
%A Sivaratnam, Gayatri
%D 2018
%T Images from Chandler, Chari et al. 2017 PLoS Genetics "How well do you know your mutation? Complex effects of genetic background on expressivity, complementation, and ordering of allelic effects"
%U https://figshare.com/articles/dataset/Images_from_Chandler_Chari_et_al_2017_PLoS_Genetics_How_well_do_you_know_your_mutation_Complex_effects_of_genetic_background_on_expressivity_complementation_and_ordering_of_allelic_effects_/5944852
%R 10.6084/m9.figshare.5944852.v1
%2 https://ndownloader.figshare.com/files/10637236
%K Drosophila melanogaster
%K Genetic Background Effects
%K images
%K morphology
%K Drosophila wings
%K scalloped
%K Genetics
%K Developmental Genetics (incl. Sex Determination)
%X These are the images associated with the data collected for
http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007075
For the zipped folder GS_data:
Data Provenance (how data was collected):
via dissections, area imaging
Author:
Performed - Ian Dworkin, Gayatri Sivaratnam, Samiksha Kaul
Dissections - Gayatri Sivaratnam, Samiksha Kaul
Imaging - I. Dworkin, G. Sivaratnam, Samiksha Kaul
Dates
samples Collected: July 21, 2015
Dissection: August 17 - September25, 2015
Imging: September 30, 2015 (ID, GS, SK)
(Date samples were collected, dates for imaging)
A bit about the experiment:
16 DGRP lines were crossed to 3 mutations (sd 1, sd E3, sd 58d) and a wildtype (SAM)
The effects of intermediate mutation on different lines, whether the severity of this effect varied across the different lines is being investigated.
Microscope, camera used. Leica m125 with a Leica DFC400 camera, using Leica Application Software 3.4.0. Greyscale images.
Each image is 1392 x 1040 px. Real size 2.59 x 1.93 mm
Magnification : 50X (0.5X?)
aperture about halfway across (59%)
Brightness: 68%
Gamma: 0.80
Notes:
naming convention for images: SAM/sd1/sde3/sd58d_line#_M_1/2/3/4/5/6.TIF
SAM/sd1/sde3/sd58d - either the wild type (SAM) allele for scalloped, or allele 1, E3 or 58d
%I figshare