Neither endo-, nor exo-vanilloids caused <sup>45</sup>Ca<sup>2+</sup>-influx in keratinocytes.
László Pecze
Kornélia Szabó
Márta Széll
Katalin Jósvay
Krisztián Kaszás
Erzsébet Kúsz
Tamás Letoha
János Prorok
István Koncz
András Tóth
Lajos Kemény
Csaba Vizler
Zoltán Oláh
10.1371/journal.pone.0003419.g005
https://plos.figshare.com/articles/figure/_Neither_endo_nor_exo_vanilloids_caused_45_Ca_2_influx_in_keratinocytes_/590014
<p>Primary human keratinocytes (NHEK), primary culture of DRG neurons, HaCaT keratinocytes and TRPV1 transformed HaCat keratinocytes were seeded in 96-well plates, then incubated for 10-min with different TRPV1 agonists (capsaicin, CAP; resiniferatoxin, RTX; N-arachidonoyl-dopamine, NADA; Anandamide, ANA) and combinations of agonists and antagonists (CAP or RTX+capsazepine, capZ) in the presence of <sup>45</sup>Ca<sup>2+</sup>. Cell-bound radioactivity was measured with liquid scintillation. The bars represent mean scintillation counts per minute (c.p.m.)+SD of eight parallel samples from a representative experiment repeated three times with similar results.</p>
2013-02-21 07:57:01
exo-vanilloids
caused