Supplementary Material for: Chromosome Nomenclature and Cytological Characterization of Sacred Lotus Meng Z. Hu X. Zhang Z. Li Z. Lin Q. Yang M. Yang P. Ming R. Yu Q. Wang K. 10.6084/m9.figshare.5882290.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Chromosome_Nomenclature_and_Cytological_Characterization_of_Sacred_Lotus/5882290 <p>Sacred lotus is a basal eudicot plant that has been cultivated in Asia for over 7,000 years for its agricultural, ornamental, religious, and medicinal importance. A notable characteristic of lotus is the seed longevity. Extensive endeavors have been devoted to dissect its genome assembly, including the variety China Antique, which germinated from a 1,300-year-old seed. Here, cytogenetic markers representing the 10 largest megascaffolds, which constitute approximately 70% of the lotus genome assembly, were developed. These 10 megascaffolds were then anchored to the corresponding lotus chromosomes by fluorescence in situ hybridization using these cytogenetic markers, and a set of chromosome-specific cytogenetic markers that could unambiguously identify each of the 8 chromosomes was generated. Karyotyping was conducted, and a nomenclature based on chromosomal length was established for the 8 chromosomes of China Antique. Comparative karyotyping revealed relatively conserved chromosomal structures between China Antique and 3 modern cultivars. Interestingly, significant variations in the copy number of 45S rDNA were detected between China Antique and modern cultivars. Our results provide a comprehensive view on the chromosomal structure of sacred lotus and will facilitate further studies and the genome assembly of lotus.</p> 2018-02-13 10:06:59 Chromosome nomenclature Cytological marker Fluorescence in situ hybridization Megascaffold Sacred lotus