Two amino acids of a conserved E-L-E-F-N motif in the N-terminal region of KSHV and RRV gH are essential for Eph interaction.
Anna K. Großkopf
Armin Ensser
Frank Neipel
Doris Jungnickl
Sarah Schlagowski
Ronald C. Desrosiers
Alexander S. Hahn
10.1371/journal.ppat.1006912.g002
https://plos.figshare.com/articles/figure/Two_amino_acids_of_a_conserved_E-L-E-F-N_motif_in_the_N-terminal_region_of_KSHV_and_RRV_gH_are_essential_for_Eph_interaction_/5880796
<p><b>A</b> Domain structure of KSHV and RRV gH. Multiple sequence alignment of domain I of gH of KSHV and the two RRV isolates 26–95 and 17577 (enlarged inset, numbers corresponding to KSHV gH). The EBV gH sequence is included as a reference. <b>B</b> Mutational scan of the N-terminal region of KSHV gH identifies EphA2-interacting residues. V5-tagged KSHV gH mutants were co-expressed with Flag-tagged KSHV gL. gH-V5/gL-Flag complexes were immunoprecipitated in the presence of full-length EphA2-myc using monoclonal antibody to the V5-tag and precipitates were analyzed by Western blot. KSHV gH alone serves as negative control. <b>C</b> Mutational scan of the N-terminal region of RRV gH identifies EphB3-interacting residues. V5-tagged gH mutants were co-expressed with Flag-tagged RRV gL. gH-V5/gL-Flag complexes were immunoprecipitated in the presence of full-length EphB3-myc using monoclonal antibody to the V5-tag and precipitates were analyzed by Western blot. RRV gH alone serves as negative control. Residues in the conserved E-L-E-F-N motif that are critical for Eph interaction are indicated by black lines. Asterisks indicate non-specific bands. Abbreviations: D: domain, TM: transmembrane domain, SP: signal peptide, IVD: intravirion domain, IP: immunoprecipitation, IB: immunoblotting.</p>
2018-02-12 18:46:56
Eph family receptor tyrosine kinases
KSHV strains
gH
protein-protein interactions
oncogenic virus
Eph-binding residues
Eph-binding-negative RRV mutants
rhesus monkey rhadinovirus
nonhuman primates
N-terminal domain
sequence analysis
RRV glycoprotein H
B-cell malignancies
log order
RRV gL
Eph family receptor-binding motif
entry mediators
Inhibition experiments
cell types
host factors
gL expression
cell type-specific use
Eph-interaction motif