TY - DATA T1 - CTB binding to primary human intestinal cells can be blocked by interference with fucosylated structures. PY - 2018/02/12 AU - Jakob Cervin AU - Amberlyn M. Wands AU - Anna Casselbrant AU - Han Wu AU - Soumya Krishnamurthy AU - Aleksander Cvjetkovic AU - Johanna Estelius AU - Benjamin Dedic AU - Anirudh Sethi AU - Kerri-Lee Wallom AU - Rebecca Riise AU - Malin Bäckström AU - Ville Wallenius AU - Frances M. Platt AU - Michael Lebens AU - Susann Teneberg AU - Lars Fändriks AU - Jennifer J. Kohler AU - Ulf Yrlid UR - https://plos.figshare.com/articles/figure/CTB_binding_to_primary_human_intestinal_cells_can_be_blocked_by_interference_with_fucosylated_structures_/5880793 DO - 10.1371/journal.ppat.1006862.g005 L4 - https://ndownloader.figshare.com/files/10579042 KW - jejunal epithelial cells KW - intoxicates host cells KW - binding cell surface receptors KW - Le X KW - detoxified enterotoxin-based adjuvants KW - GM 1 KW - Cholera toxin Cholera toxin KW - fucosylated Lewis X KW - GM 1-deficient C 6 rat glioma cells results KW - block CT action KW - protein KW - Le X glycan KW - glycosphingolipid KW - flow cytometric analysis KW - CTB binding correlates KW - gm 1 ganglioside-independent intoxication N2 - (A) Bar graph showing relative absorbance values from an ELISA with immobilized anti-LeX, and detection with CTB-HRP. Samples as indicated from lysates of isolated human cells (2 μg protein/ml). Each dot represents a human donor (n = 5–8). (B) CD66 or (C) CD66 and LeX expression by jejunal epithelial cells that were isolated using EDTA medium (villi) or enzymatic degradation after EDTA treatment (non-villi or crypt). Histograms from flow cytometry analyses of CTB-, G33D- and OVA-binding to the differentially enriched epithelial cells. (B) EpCAM+ cells and (C) EpCAM+LeX+ cells. (D-G) Bar graph showing percent of gMFI of CTB binding to jejunal epithelial cells by pretreatment of the cells with (D) lectins, (E) sugars, (F) oligosaccharides and (G) HSA-linked oligosaccharides. Graphs show the percent of gMFI of CTB binding to the cells where 100% represents CTB staining with no blocking oligosaccharide. Each dot represents a donor in (D) n = 4–12, (E) n = 6–8, (F) n = 6–12, (G) n = 6–7. Significance was calculated using a one-way-ANOVA with Tukey correction compared to CTB without block if not indicated otherwise with bars (**** = p<0,0001, *** = p<0,005, ** = p<0,01 and * = p<0,05). ER -