10.1371/journal.pone.0192884.g002 Hiroyuki Sanjo Hiroyuki Sanjo Mitsuru Komeya Mitsuru Komeya Takuya Sato Takuya Sato Takeru Abe Takeru Abe Kumiko Katagiri Kumiko Katagiri Hiroyuki Yamanaka Hiroyuki Yamanaka Yoko Ino Yoko Ino Noriaki Arakawa Noriaki Arakawa Hisashi Hirano Hisashi Hirano Tatsuma Yao Tatsuma Yao Yuta Asayama Yuta Asayama Akio Matsuhisa Akio Matsuhisa Masahiro Yao Masahiro Yao Takehiko Ogawa Takehiko Ogawa Effects of retinoic acid and lipids. Public Library of Science 2018 Knockout serum replacement KSR FSH mouse spermatogenesis testis organ culture organ culture method round spermatid production LH RA medium organ culture conditions 2 days postpartum MEM CDM BSA 2018-02-12 18:45:26 Figure https://plos.figshare.com/articles/figure/Effects_of_retinoic_acid_and_lipids_/5880742 <p><b>A)</b> Representative <i>Acr</i>-GFP expression grade time-course with the addition of RA and retinol (RA, Re), lipids, and both in the medium is shown in the graph. Asterisk indicates P<0.01. <b>B)</b> Histological examination with standard H.E. staining, and immunohistochemistry with antibodies for γH2AX and Sycp1 counterstained with Hoechst. Sycp1-positive cells, indicated by arrows, were pachytene-stage spermatocytes. Scale bar: 50 γm.</p>