10.1371/journal.pone.0192884
Hiroyuki Sanjo
Hiroyuki
Sanjo
Mitsuru Komeya
Mitsuru
Komeya
Takuya Sato
Takuya
Sato
Takeru Abe
Takeru
Abe
Kumiko Katagiri
Kumiko
Katagiri
Hiroyuki Yamanaka
Hiroyuki
Yamanaka
Yoko Ino
Yoko
Ino
Noriaki Arakawa
Noriaki
Arakawa
Hisashi Hirano
Hisashi
Hirano
Tatsuma Yao
Tatsuma
Yao
Yuta Asayama
Yuta
Asayama
Akio Matsuhisa
Akio
Matsuhisa
Masahiro Yao
Masahiro
Yao
Takehiko Ogawa
Takehiko
Ogawa
<i>In vitro</i> mouse spermatogenesis with an organ culture method in chemically defined medium
Public Library of Science
2018
Knockout serum replacement
KSR
FSH
mouse spermatogenesis
testis organ culture
organ culture method
round spermatid production
LH
RA
medium
organ culture conditions
2 days postpartum
MEM
CDM
BSA
2018-02-12 18:45:26
Dataset
https://plos.figshare.com/articles/dataset/_i_In_vitro_i_mouse_spermatogenesis_with_an_organ_culture_method_in_chemically_defined_medium/5880724
<div><p>We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or AlbuMAX, neither of which were defined chemically. In this study, we formulated a chemically defined medium (CDM) that can induce mouse spermatogenesis under organ culture conditions. It was found that bovine serum albumin (BSA) purified through three different procedures had different effects on spermatogenesis. We also confirmed that retinoic acid (RA) played crucial roles in the onset of spermatogonial differentiation and meiotic initiation. The added lipids exhibited weak promoting effects on spermatogenesis. Lastly, luteinizing hormone (LH), follicle stimulating hormone (FSH), triiodothyronine (T3), and testosterone (T) combined together promoted spermatogenesis until round spermatid production. The CDM, however, was not able to produce elongated spermatids. It was also unable to induce spermatogenesis from the very early neonatal period, before 2 days postpartum, leaving certain factors necessary for spermatogenic induction in mice unidentified. Nonetheless, the present study provided important basic information on testis organ culture and spermatogenesis <i>in vitro</i>.</p></div>