10.4225/03/5a825d649f7c7 SACHITH DILSHAN GUNASINGHE SACHITH DILSHAN GUNASINGHE A Single Molecule Localization and In situ Crosslinking Study of the Beta-barrel Assembly Machinery in Escherichia coli Monash University 2019 Super-resolution Microscopy Microbiology Biochemistry Molecular Biology Microbiology Biochemistry Molecular Biology 2019-03-27 21:11:47 Thesis https://bridges.monash.edu/articles/thesis/A_Single_Molecule_Localization_and_In_situ_Crosslinking_Study_of_the_Beta-barrel_Assembly_Machinery_in_Escherichia_coli/5872458 In order to sense changes in, respond to, and exploit their environmental niches, bacteria rely on structures assembled into their outer membrane. A set of bacterial proteins collectively known as the Beta-barrel Assembly Machinery is solely responsible for catalysing the biogenesis of this membrane. By employing genetic code expansion tools and advanced molecular imaging techniques, the Super-resolution microscopy, we interrogated the interactome and membrane spatial representation of these proteins. Our findings present a missing piece of the mechanistic puzzle on how these proteins catalyse the events involving the propagation of the outer membrane.