Detection of pullulanase activity in GBS total extracts. Isabella Santi Alfredo Pezzicoli Mattia Bosello Francesco Berti Massimo Mariani John L. Telford Guido Grandi Marco Soriani 10.1371/journal.pone.0003787.g007 https://plos.figshare.com/articles/figure/_Detection_of_pullulanase_activity_in_GBS_total_extracts_/586361 <p>Bacteria grown in a complex medium supplemented with the indicated sugars were used to prepare total extracts. Bacterial extracts were then incubated with pullulan and pullulanase enzymatic activity measured by DNS acid assay. The asterisks indicate a significant difference between the activity of the wild type strain versus the mutant strain derived extracts (p<0,01). The data are the mean of 3 independent experiments ± SD.</p> 2013-02-21 07:36:04 pullulanase gbs