Structures and activity in fluorometric assays of HC-070 and HC-608. JustStefan ChenardBertrand L. CeciAngelo StrassmaierTimothy A. ChongJayhong BlairNathaniel T. GallaschunRandall J. del CaminoDonato CantinSusan D’AmoursMarc EickmeierChristian M. FangerChristopher HeckerCarsten HesslerDavid P. HengererBastian KrokerKatja S. MalekianiSam MihalekRobert McLaughlinJoseph RastGeorg WitekJoAnn SauerAchim PryceChristopher R. M. MoranMagdalene 2018 <p>The chemical structures of (A) HC-070 and (B) HC-608 (Pico145). (C) Inhibition of hTRPC5 by HC-070 and HC-608 in indicator-assisted calcium influx analysis. Concentrations tested ranged from 1 picomolar (pM) to 1 μM. Each data point represents the average of 8 measurements from a 384-well plate. Error bars show the standard deviation. The IC<sub>50</sub> values were 9.3 ± 0.9 and 6.2 ± 0.5 nanomolar (nM), respectively. (D) Inhibition of hTRPC4 by HC-070 and HC-608 over the same range of concentrations. The IC<sub>50</sub> values were 46.0 ± 3.9 nM and 32.5 ± 1.8 nM, respectively (n = 8). Error bars represent the standard deviation.</p>