10.1371/journal.pone.0191667.g001
Min-Yin Guo
Min-Yin
Guo
Hua Wang
Hua
Wang
Yuan-Hua Chen
Yuan-Hua
Chen
Mi-Zhen Xia
Mi-Zhen
Xia
Cheng Zhang
Cheng
Zhang
De-Xiang Xu
De-Xiang
Xu
Effects of NAC on Cd-induced impairment of placental vascular space.
Public Library of Science
2018
placental ER stress
GD
cadmium-induced placental endoplasmic reticulum stress
placental cell proliferation
placental growth factor
Cd-induced placental insufficiency
NAC alleviated Cd-induced FGR
growth restriction
Placental endoplasmic reticulum
Cd-induced placental ER stress
placental labyrinth layer
2018-01-26 18:32:12
Figure
https://plos.figshare.com/articles/figure/Effects_of_NAC_on_Cd-induced_impairment_of_placental_vascular_space_/5828895
<p>All pregnant mice except controls were i.p. injected with CdCl<sub>2</sub> (1.0 mg/kg) daily from GD13 to GD17. In NAC+Cd group, pregnant mice were i.p. injected with NAC (1.0 g/kg) daily from GD12 to GD17, one dose (300 mg/kg) at 8 h before CdCl<sub>2</sub> injection, one (400 mg/kg) at 30 min before CdCl<sub>2</sub> injection, another (300 mg/kg) at 8 h after CdCl<sub>2</sub> injection. Placentas were collected on GD 18. (A) Placental cross sections were stained with H & E. Original magnification: 200×. (B) Vascular area in the labyrinthine layer was estimated from two nonconsecutive sections in each placenta using the public domain NIH Image J Program. The blood sinusoid area (%) was calculated as the ratio between the number of pixels covered by the area defined by the threshold and the overall number of pixels in the image. (C) Placental CD34 was analyzed using immunohistochemistry. Representative photomicrographs of histological specimens in the labyrinthine layer among different groups. Original magnification: 200×. Arrows indicate CD34-positive blood vessels. The images using black line were captured at magnification of 400×. (D) CD34-positive blood vessels were counted. (B and D) All data were expressed as means ± SEM. (N = 14~20). * <i>P</i><0.05, **<i>P</i><0.01.</p>