10.1371/journal.pone.0191621.g005
Julieta B. Carrillo
Julieta B.
Carrillo
Diego F. Gomez-Casati
Diego
F. Gomez-Casati
Mariana Martín
Mariana
Martín
Maria V. Busi
Maria
V. Busi
Affinity gel electrophoresis (AGE) of OsttaDSPwild-type (WT) and C162S mutated protein (C162S).
Public Library of Science
2018
starch metabolism exhibits
glycogen degradation
electrophoretic homogeneity
Ostreococcus tauri Ostreococcus tauri
Arabidopsis thaliana LSF 2.
enzyme forms
Prasinophyceae family
residue C 162
dephosphorylates amylopectin
glucan phosphatases
phosphoglucan phosphatase
tauri microalgae
OsttaDSP
Glucan phosphatases
gene coding
54.5 kDa
starch granule
heterologous expression
2018-01-23 18:33:23
Figure
https://plos.figshare.com/articles/figure/Affinity_gel_electrophoresis_AGE_of_OsttaDSPwild-type_WT_and_C162S_mutated_protein_C162S_/5815080
<p>OsttaDSP WT (WT, 5 μg) and OsttaDSP C162S (C162S, 5 μg) proteins were run on native PAGE without or with amylopectin (0.3 mg/ml) simultaneously under the same conditions. Gels were then revealed by Coomassie Blue staining. The number indicates the molecular mass marker in kDa. MMM, molecular mass markers (Amersham High Molecular Weight Calibration Kit (range 66–669 kDa)).</p>