10.1371/journal.pone.0191621.g005 Julieta B. Carrillo Julieta B. Carrillo Diego F. Gomez-Casati Diego F. Gomez-Casati Mariana Martín Mariana Martín Maria V. Busi Maria V. Busi Affinity gel electrophoresis (AGE) of OsttaDSPwild-type (WT) and C162S mutated protein (C162S). Public Library of Science 2018 starch metabolism exhibits glycogen degradation electrophoretic homogeneity Ostreococcus tauri Ostreococcus tauri Arabidopsis thaliana LSF 2. enzyme forms Prasinophyceae family residue C 162 dephosphorylates amylopectin glucan phosphatases phosphoglucan phosphatase tauri microalgae OsttaDSP Glucan phosphatases gene coding 54.5 kDa starch granule heterologous expression 2018-01-23 18:33:23 Figure https://plos.figshare.com/articles/figure/Affinity_gel_electrophoresis_AGE_of_OsttaDSPwild-type_WT_and_C162S_mutated_protein_C162S_/5815080 <p>OsttaDSP WT (WT, 5 μg) and OsttaDSP C162S (C162S, 5 μg) proteins were run on native PAGE without or with amylopectin (0.3 mg/ml) simultaneously under the same conditions. Gels were then revealed by Coomassie Blue staining. The number indicates the molecular mass marker in kDa. MMM, molecular mass markers (Amersham High Molecular Weight Calibration Kit (range 66–669 kDa)).</p>