10.6084/m9.figshare.5809458.v1 Keng Hong Tee Keng Hong Tee Gwendoline Cheng Lian Ee Gwendoline Cheng Lian Ee Intan Safinar Ismail Intan Safinar Ismail Thiruventhan Karunakaran Thiruventhan Karunakaran Soek Sin Teh Soek Sin Teh Vivien Yi Mian Jong Vivien Yi Mian Jong Siti Mariam Mohd Nor Siti Mariam Mohd Nor A new coumarin from stem bark of <i>Calophyllum wallichianum</i> Taylor & Francis Group 2018 Calophyllum wallichianum coumarin anti-bacterial 2018-01-22 12:48:40 Journal contribution https://tandf.figshare.com/articles/journal_contribution/A_new_coumarin_from_stem_bark_of_i_Calophyllum_wallichianum_i_/5809458 <p>A phytochemical study carried out on the plant, <i>Calophyllum wallichianum</i> has led to the isolation of a new coumarin, wallimarin T (<b>1</b>) and a known coumarin, calanolide E (<b>2</b>) along with two common triterpenes, friedelin (<b>3</b>) and stigmasterol (<b>4</b>). The structures of these compounds were elucidated with the aid of spectroscopic analyses such as FT-IR, GC-MS, and NMR. MIC assay against the <i>Bacillus</i> bacteria were conducted on the extracts and this gave MIC values ranging from 0.313 to 1.25 mg/mL. Compound <b>2</b> was weakly inhibitory towards the <i>Bacilli</i> strains with MIC values ranging from 0.25–0.50 mg/mL. Wallimarin T <b>(1)</b> was not active towards all four bacteria. Overall, the extracts exhibited weak bactericidal properties whereas compound <b>2</b> was not bactericidal on the tested bacteria. The hexane and chloroform extracts of the plant were found to be inhibitors to the growth of <i>Bacillus megaterium</i>, <i>Bacillus cereus</i>, <i>Bacillus pumilus</i> and <i>Bacillus subtilis</i>.</p>