10.1371/journal.ppat.1006834.g003
Chunhong Yin
Chunhong
Yin
Niluka Goonawardane
Niluka
Goonawardane
Hazel Stewart
Hazel
Stewart
Mark Harris
Mark
Harris
Density gradient analysis of mutant viruses.
Public Library of Science
2018
JFH -1-derived sub-genomic replicon
genome replication
HCV
lipid droplets
NS 5A domain
UTR
P 35A V 67A
virus genome replication
virus assembly
hepatitis C virus NS 5A protein
hepatitis C virus
V 67A
NS 5A
RNA
density gradient fractionation
NS 5A protein
P 145A
lipid droplet morphology
III
resolution confocal microscopy
II
2018-01-19 19:05:42
Figure
https://plos.figshare.com/articles/figure/Density_gradient_analysis_of_mutant_viruses_/5806860
<p>Huh7.5 cells were electroporated with <i>in vitro</i> transcripts of WT or the indicated virus mutants. Concentrated culture medium was fractionated using 10–40% iodixanol density-gradient centrifugation. For each fraction, HCV RNA (<b>A</b>) and infectivity (<b>B</b>) were plotted against the buoyant density (n = 3), and Core protein in each fraction was detected by western blot (C). 1 to 12 in (C) indicated the fractions collected from top to bottom with the buoyant density indicated in (A) and (<b>B</b>). The result of a representative of three independent experiments is shown.</p>