10.1371/journal.ppat.1006834.g003 Chunhong Yin Chunhong Yin Niluka Goonawardane Niluka Goonawardane Hazel Stewart Hazel Stewart Mark Harris Mark Harris Density gradient analysis of mutant viruses. Public Library of Science 2018 JFH -1-derived sub-genomic replicon genome replication HCV lipid droplets NS 5A domain UTR P 35A V 67A virus genome replication virus assembly hepatitis C virus NS 5A protein hepatitis C virus V 67A NS 5A RNA density gradient fractionation NS 5A protein P 145A lipid droplet morphology III resolution confocal microscopy II 2018-01-19 19:05:42 Figure https://plos.figshare.com/articles/figure/Density_gradient_analysis_of_mutant_viruses_/5806860 <p>Huh7.5 cells were electroporated with <i>in vitro</i> transcripts of WT or the indicated virus mutants. Concentrated culture medium was fractionated using 10–40% iodixanol density-gradient centrifugation. For each fraction, HCV RNA (<b>A</b>) and infectivity (<b>B</b>) were plotted against the buoyant density (n = 3), and Core protein in each fraction was detected by western blot (C). 1 to 12 in (C) indicated the fractions collected from top to bottom with the buoyant density indicated in (A) and (<b>B</b>). The result of a representative of three independent experiments is shown.</p>