IFNγ controls neutrophil responses via apoptosis and SOCS3 expression. RamakrishnaChandran M. CantinEdouard 2018 <p><b>(A)</b> Relative SOCS3 expression from Ly6G<sup>+</sup> neutrophils (PMN) isolated from BM and blood (Bld) of WT and GKO mice at day 6 pi, as measured by RT-PCR; levels are relative to PMN isolated from uninfected mice. Blood-derived PMN treated with recombinant IFNγ in vitro for 30 min were compared to untreated neutrophils for SOCS3 expression. Data are representative of 2–3 experiments; *p = 0.013; **p = 0.0035. <b>(B)</b> Bar plots depict Annexin V<sup>+</sup> PMN in the blood of WT and GKO mice at days 6 and 8 pi and BS at day 8 pi. Data are representative of 2 experiments with 2–3 mice per group. **p = 0.0011. (<b>C & D</b>) FACS plots show intracellular staining for IL-10 (left plots) and IL-17 (right plots) by γδ T cells isolated from <b>(C)</b> CLN and <b>(D)</b> spleen of GKO mice at day 14 pi. Red dots indicate no antigenic stimulation; blue dots indicate cells stimulated with PMA + ionomycin + HK-HSV.</p>