10.1371/journal.ppat.1006822.g008
Chandran Ramakrishna
Chandran
Ramakrishna
Edouard M. Cantin
Edouard
M. Cantin
IFNγ controls neutrophil responses via apoptosis and SOCS3 expression.
Public Library of Science
2018
WT
HSE
GKO mice
neutrophils
IFN
G-CSF-IFN
GM-CSF
IL -10 secretion
HSV 1 encephalitis
infection
encephalitis Emergency hematopoiesis
G-CSF
SOCS 3 expression
CNS
response
anti-inflammatory cytokine IL -10. Unexpectedly
2018-01-19 19:05:21
Figure
https://plos.figshare.com/articles/figure/IFN_controls_neutrophil_responses_via_apoptosis_and_SOCS3_expression_/5806848
<p><b>(A)</b> Relative SOCS3 expression from Ly6G<sup>+</sup> neutrophils (PMN) isolated from BM and blood (Bld) of WT and GKO mice at day 6 pi, as measured by RT-PCR; levels are relative to PMN isolated from uninfected mice. Blood-derived PMN treated with recombinant IFNγ in vitro for 30 min were compared to untreated neutrophils for SOCS3 expression. Data are representative of 2–3 experiments; *p = 0.013; **p = 0.0035. <b>(B)</b> Bar plots depict Annexin V<sup>+</sup> PMN in the blood of WT and GKO mice at days 6 and 8 pi and BS at day 8 pi. Data are representative of 2 experiments with 2–3 mice per group. **p = 0.0011. (<b>C & D</b>) FACS plots show intracellular staining for IL-10 (left plots) and IL-17 (right plots) by γδ T cells isolated from <b>(C)</b> CLN and <b>(D)</b> spleen of GKO mice at day 14 pi. Red dots indicate no antigenic stimulation; blue dots indicate cells stimulated with PMA + ionomycin + HK-HSV.</p>