10.1371/journal.ppat.1006822.g008 Chandran Ramakrishna Chandran Ramakrishna Edouard M. Cantin Edouard M. Cantin IFNγ controls neutrophil responses via apoptosis and SOCS3 expression. Public Library of Science 2018 WT HSE GKO mice neutrophils IFN G-CSF-IFN GM-CSF IL -10 secretion HSV 1 encephalitis infection encephalitis Emergency hematopoiesis G-CSF SOCS 3 expression CNS response anti-inflammatory cytokine IL -10. Unexpectedly 2018-01-19 19:05:21 Figure https://plos.figshare.com/articles/figure/IFN_controls_neutrophil_responses_via_apoptosis_and_SOCS3_expression_/5806848 <p><b>(A)</b> Relative SOCS3 expression from Ly6G<sup>+</sup> neutrophils (PMN) isolated from BM and blood (Bld) of WT and GKO mice at day 6 pi, as measured by RT-PCR; levels are relative to PMN isolated from uninfected mice. Blood-derived PMN treated with recombinant IFNγ in vitro for 30 min were compared to untreated neutrophils for SOCS3 expression. Data are representative of 2–3 experiments; *p = 0.013; **p = 0.0035. <b>(B)</b> Bar plots depict Annexin V<sup>+</sup> PMN in the blood of WT and GKO mice at days 6 and 8 pi and BS at day 8 pi. Data are representative of 2 experiments with 2–3 mice per group. **p = 0.0011. (<b>C & D</b>) FACS plots show intracellular staining for IL-10 (left plots) and IL-17 (right plots) by γδ T cells isolated from <b>(C)</b> CLN and <b>(D)</b> spleen of GKO mice at day 14 pi. Red dots indicate no antigenic stimulation; blue dots indicate cells stimulated with PMA + ionomycin + HK-HSV.</p>