10.1021/acs.langmuir.7b04003.s001 Roobala Chelladurai Roobala Chelladurai Koushik Debnath Koushik Debnath Nikhil R. Jana Nikhil R. Jana Jaydeep Kumar Basu Jaydeep Kumar Basu Nanoscale Heterogeneities Drive Enhanced Binding and Anomalous Diffusion of Nanoparticles in Model Biomembranes American Chemical Society 2018 fluorescence correlation spectroscopy nanoparticle binding subdiffraction nanoscale dynamics Model Biomembranes Interaction membrane nanoscale platform STED-FCS drug delivery vehicles understanding nanoparticle cytotoxicity effects nanoparticle diffusion FCS model biomembranes STED tunable surface charge cell membranes Nanoscale Heterogeneities Drive Enhanced Binding 2018-01-10 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Nanoscale_Heterogeneities_Drive_Enhanced_Binding_and_Anomalous_Diffusion_of_Nanoparticles_in_Model_Biomembranes/5803158 Interaction of functional nanoparticles with cells and model biomembranes has been widely studied to evaluate the effectiveness of the particles as potential drug delivery vehicles and bioimaging labels as well as in understanding nanoparticle cytotoxicity effects. Charged nanoparticles, in particular, with tunable surface charge have been found to be effective in targeting cellular membranes as well as the subcellular matrix. However, a microscopic understanding of the underlying physical principles that govern nanoparticle binding, uptake, or diffusion on cells is lacking. Here, we report the first experimental studies of nanoparticle diffusion on model biomembranes and correlate this to the existence of nanoscale dynamics and structural heterogeneities using super-resolution stimulated emission depletion (STED) microscopy. Using confocal and STED microscopy coupled with fluorescence correlation spectroscopy (FCS), we provide novel insight on why these nanoparticles show enhanced binding on two-component lipid bilayers as compared to single-component membranes and how binding and diffusion is correlated to subdiffraction nanoscale dynamics and structure. The enhanced binding is also dictated, in part, by the presence of structural and dynamic heterogeneity, as revealed by STED-FCS studies, which could potentially be used to understand enhanced nanoparticle binding in raft-like domains in cell membranes. In addition, we also observe a clear correlation between the enhanced nanoparticle diffusion on membranes and the extent of membrane penetration by the nanoparticles. Our results not only have a significant impact on our understanding of nanoparticle binding and uptake as well as diffusion in cell and biomembranes, but have very strong implications for uptake mechanisms and diffusion of other biomolecules, like proteins on cell membranes and their connections to functional membrane nanoscale platform.