10.6084/m9.figshare.5792916.v1 Patricia J. Ward Patricia J. Ward Scott Lee Clanton Scott Lee Clanton Arthur W. English Arthur W. English Dataset for: Optogenetically-enhanced Axon Regeneration: Motor- versus Sensory- Neuron Specific Stimulation Wiley 2018 Channel rhodopsin DRG neuron cell autonomous neuronal activity Neuroscience 2018-02-10 06:47:00 Dataset https://wiley.figshare.com/articles/dataset/Dataset_for_Optogenetically-enhanced_Axon_Regeneration_Motor-_versus_Sensory-_Neuron_Specific_Stimulation/5792916 Brief neuronal activation in injured peripheral nerves is both necessary and sufficient to enhance motor axon regeneration, and this effect is specific to the activated motoneurons. It is less clear whether sensory neurons respond in a similar manner to neuronal activation following peripheral axotomy. Further, it is unknown to what extent enhancement of axon regeneration with increased neuronal activity relies on a reflexive interaction within the spinal circuitry. We used mouse genetics and optical tools to evaluate the precision and selectivity of system-specific neuronal activation to enhance axon regeneration in a mixed nerve. We evaluated sensory and motor axon regeneration in two different mouse models expressing the light-sensitive cation channel, channel rhodopsin (ChR2). We selectively activated either sensory or motor axons using light stimulation and transected and repaired the sciatic nerve. Regardless of genotype, the number of ChR2-positive neurons whose axons had regenerated successfully was greater following system-specific optical treatment, with no effect on the number of ChR2-negative neurons (whether motor or sensory neurons). We conclude that acute system-specific neuronal activation is sufficient to enhance both motor and sensory axon regeneration. This regeneration-enhancing effect is likely cell autonomous.