Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification V. Coimbra-e-Souza M.A.V.P. Brito R.C. Chamon M.S. Laport M. Giambiagi-deMarval 10.6084/m9.figshare.5792514.v1 https://scielo.figshare.com/articles/figure/Characterization_of_Staphylococcus_spp_strains_in_milk_from_buffaloes_with_mastitis_in_Brazil_the_need_to_identify_to_species_level_to_avoid_misidentification/5792514 <div><p>ABSTRACT Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and coagulase-positive Staphylococcus aureus (2). Our results strongly support the need to identify the isolates to a species level in order to avoid misidentification and to be aware of the classification using the coagulase test alone.</p></div> 2018-01-17 02:54:19 buffalo mastitis Staphylococcus milk coagulase test