TY - DATA T1 - Cerebrospinal fluid cytological and biochemical values of healthy cattle in two sampling times with an interval of 96 hours PY - 2018/01/17 AU - P.H.J. Cunha AU - D.G.A. Andrade AU - J.P. Oliveira-Filho AU - P.R. Badial AU - D. Passarelli AU - R.K. Takahira AU - J.A.N. Lisboa AU - A.S. Borges UR - https://scielo.figshare.com/articles/dataset/Cerebrospinal_fluid_cytological_and_biochemical_values_of_healthy_cattle_in_two_sampling_times_with_an_interval_of_96_hours/5792274 DO - 10.6084/m9.figshare.5792274.v1 L4 - https://ndownloader.figshare.com/files/10227723 L4 - https://ndownloader.figshare.com/files/10227726 KW - cattle KW - cerebrospinal fluid KW - inflammation N2 - ABSTRACT The analysis of cerebrospinal fluid (CSF) is important for the diagnosis of neurological diseases in cattle. However, the effect of consecutive collections on the cytological and biochemical parameters of the CSF has not been evaluated yet. The objective of the present study was to evaluate potential differences on the cytological and biochemical analysis of CSF obtained from healthy cattle at two sampling times with 96 hours of interval in between. Total and differential cell counts, and total protein concentration were analyzed in the CSF. Increased cellularity and significant reduction of lymphocyte and elevated numbers of mononuclear cells were observed on the second collection. However, erythrocyte count (/µl), total leukocyte count (/µl), neutrophils (%), eosinophils (%) and total protein concentration (mg/dl) remained within the reference range 96 hours after the completion of the first puncture. In conclusion, consecutive CSF collections cause no detectable local inflammation 96 hours after the first puncture suggesting that repeated collections do not interfere in the CSF results. ER -