10.1371/journal.pone.0190158
James Ziai
James
Ziai
Houston N. Gilbert
Houston N.
Gilbert
Oded Foreman
Oded
Foreman
Jeffrey Eastham-Anderson
Jeffrey
Eastham-Anderson
Felix Chu
Felix
Chu
Mahrukh Huseni
Mahrukh
Huseni
Jeong M. Kim
Jeong M.
Kim
CD8+ T cell infiltration in breast and colon cancer: A histologic and statistical analysis
Public Library of Science
2018
FFPE
TIL
results show
CD 8 immunohistochemistry
cytotoxic T cell infiltration
cytotoxic T cell prevalence
tissue sample
marker variability
histologic sections
ROI
CD 45RO
findings support
cytotoxic tumor
biopsy fragments
CD 3
UICC
colorectal cancer samples
tissue samples
chromogenic immunohistochemistry
marker prevalence
biomarker strategies
breast carcinomas
tumor types
T cell biomarker assessment
CD 8 counts
prognostic value
breast cancer patients
patient sample
colon cancer patients
tumor section
step sections
colon cancer
Statistical analyses
2018-01-10 18:33:39
Dataset
https://plos.figshare.com/articles/dataset/CD8_T_cell_infiltration_in_breast_and_colon_cancer_A_histologic_and_statistical_analysis/5775315
<div><p>The prevalence of cytotoxic tumor infiltrating lymphocytes (TILs) has demonstrated prognostic value in multiple tumor types. In particular, CD8 counts (in combination with CD3 and CD45RO) have been shown to be superior to traditional UICC staging in colon cancer patients and higher total CD8 counts have been associated with better survival in breast cancer patients. However, immune infiltrate heterogeneity can lead to potentially significant misrepresentations of marker prevalence in routine histologic sections. We examined step sections of breast and colorectal cancer samples for CD8+ T cell prevalence by standard chromogenic immunohistochemistry to determine marker variability and inform practice of T cell biomarker assessment in formalin-fixed, paraffin-embedded (FFPE) tissue samples. Stained sections were digitally imaged and CD8+ lymphocytes within defined regions of interest (ROI) including the tumor and surrounding stroma were enumerated. Statistical analyses of CD8+ cell count variability using a linear model/ANOVA framework between patients as well as between levels within a patient sample were performed. Our results show that CD8+ T-cell distribution is highly homogeneous within a standard tissue sample in both colorectal and breast carcinomas. As such, cytotoxic T cell prevalence by immunohistochemistry on a single level or even from a subsample of biopsy fragments taken from that level can be considered representative of cytotoxic T cell infiltration for the entire tumor section within the block. These findings support the technical validity of biomarker strategies relying on CD8 immunohistochemistry.</p></div>