10.6084/m9.figshare.5766213.v1 Maryam Akhbari Maryam Akhbari Reza Kord Reza Kord Saeedeh Jafari Nodooshan Saeedeh Jafari Nodooshan Sepideh Hamedi Sepideh Hamedi Analysis and evaluation of the antimicrobial and anticancer activities of the essential oil isolated from <i>Foeniculum vulgare</i> from Hamedan, Iran Taylor & Francis Group 2018 Foeniculum vulgare biological activity antibacterial evaluation anticancer activity 2018-01-08 07:01:43 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Analysis_and_evaluation_of_the_antimicrobial_and_anticancer_activities_of_the_essential_oil_isolated_from_i_Foeniculum_vulgare_i_from_Hamedan_Iran/5766213 <p>In this study, biological properties of the essential oil isolated from seeds of <i>Foeniculum vulgare</i> (<i>F. vulgare</i>) were evaluated. GC-MS analysis revealed Trans-Anethole (80.63%), L-Fenchone (11.57%), Estragole (3.67%) and Limonene (2.68%) were the major compounds of the essential oil. Antibacterial activity of the essential oil against nine Gram-positive and Gram-negative strains was studied using disc diffusion and micro-well dilution assays. Essential oil exhibited the antibacterial activity against three Gram-negative strains of <i>Pseudomonas aeruginosa</i>, <i>Escherichia coli</i>, and <i>Shigella dysenteriae</i>. The preliminary study on toxicity of seed oil was performed using Brine Shrimp lethality test (BSLT). Results indicated the high toxicity effect of essential oil (LC50 = 10 μg/mL). <i>In vitro</i> anticancer activity of seed oil was investigated against human breast cancer (MDA-Mb) and cervical epithelioid carcinoma (Hela) cell lines by MTT assay. Results showed the seed oil behave as a very potent anticancer agent with IC50 of lower than 10 μg/mL in both cases.</p>