Endogenous R232H STING is partly deficient in sensing bacterial CDN but responds normally to <i>Legionella</i> infection or stimulation with DNA.
Juan S. Ruiz-Moreno
Lutz Hamann
Javeed A. Shah
Annelies Verbon
Frank P. Mockenhaupt
Monika Puzianowska-Kuznicka
Jan Naujoks
Leif E. Sander
Martin Witzenrath
John C. Cambier
Norbert Suttorp
Ralf R. Schumann
Lei Jin
Thomas R. Hawn
Bastian Opitz
10.1371/journal.ppat.1006829.g005
https://plos.figshare.com/articles/figure/Endogenous_R232H_STING_is_partly_deficient_in_sensing_bacterial_CDN_but_responds_normally_to_i_Legionella_i_infection_or_stimulation_with_DNA_/5755266
<p>(A-D) PBMCs from healthy volunteers (N = 3 for WT and N = 3 for R232H) were isolated by density gradient centrifugation. 7 d after isolation cells were infected for 6 h with <i>L</i>. <i>pneumophila</i> at MOI 10 or stimulated for the same period with 1 ug/ml 2´-3´cGAMP, Rp-c-diAMPSS, cGMP or either bacterial DNA at a concentration of 1 ug/ml. RNA was isolated and the expression of <i>IFNB</i> (A), <i>IL1B</i> (B), and <i>IL6</i> (C) and <i>TNFA</i> (D) was determined by qRT-PCR. Data are shown as the RQ of specified mRNAs. Data represent the mean ± SEM of 3 independent experiments carried out in triplicates. Differences were assessed with the Mann-Whitney U Test. Comparisons with a p < 0.05 were considered significant.</p>
2018-01-03 18:40:52
R 232H attenuated cytokine production
susceptibility
STING-deficient murine macrophages
R 232H alleles
cyclic GMP-AMP synthase
STING-deficient animals
cyclic dinucleotides
TMEM
IFN
protozoal infections
Recent studies
HAQ STING variant impairs cGAS-dependent
cGAS-STING cascade
Legionnaire
haplotype frequency
DNA
Trial registration ClinicalTrials.gov DRKS 00005274
CDN
mouse model
wild-type mice
Legionella pneumophila
response
pro-inflammatory cytokines