TY - DATA T1 - The cGAS/STING axis contributes to the production of pro-inflammatory cytokines during L. pneumophila infection. PY - 2018/01/03 AU - Juan S. Ruiz-Moreno AU - Lutz Hamann AU - Javeed A. Shah AU - Annelies Verbon AU - Frank P. Mockenhaupt AU - Monika Puzianowska-Kuznicka AU - Jan Naujoks AU - Leif E. Sander AU - Martin Witzenrath AU - John C. Cambier AU - Norbert Suttorp AU - Ralf R. Schumann AU - Lei Jin AU - Thomas R. Hawn AU - Bastian Opitz UR - https://plos.figshare.com/articles/figure/The_cGAS_STING_axis_contributes_to_the_production_of_pro-inflammatory_cytokines_during_i_L_i_i_pneumophila_i_infection_/5755251 DO - 10.1371/journal.ppat.1006829.g002 L4 - https://ndownloader.figshare.com/files/10223451 KW - R 232H attenuated cytokine production KW - susceptibility KW - STING-deficient murine macrophages KW - R 232H alleles KW - cyclic GMP-AMP synthase KW - STING-deficient animals KW - cyclic dinucleotides KW - TMEM KW - IFN KW - protozoal infections KW - Recent studies KW - HAQ STING variant impairs cGAS-dependent KW - cGAS-STING cascade KW - Legionnaire KW - haplotype frequency KW - DNA KW - Trial registration ClinicalTrials.gov DRKS 00005274 KW - CDN KW - mouse model KW - wild-type mice KW - Legionella pneumophila KW - response KW - pro-inflammatory cytokines N2 - (A-F) WT, Tmem173-/- and cGas-/- BMDMs were infected for 6 h with L. pneumophila WT at MOI 10 and relative cytokine expression was determined by qRT-PCR. (G-J) Cytokine protein concentrations in whole lung homogenates from L. pneumophila-infected mice were quantified by sandwich ELISA. Data are shown as mean ± SEM. (A-F) Data representative of 3 to 4 independent experiments carried out in duplicates. (G-J) Data representative of 6 o 7 mice per group. Data were analyzed through the Mann-Whitney U Test. Comparisons with a p < 0.05 were considered significant. ER -