Siegel, David Dehn, Donna D. Bokatzian, Samantha S. Quinn, Kevin Backos, Donald S. Di Francesco, Andrea Bernier, Michel Reisdorph, Nichole de Cabo, Rafael Ross, David Intracellular oxidation of pyridine nucleotides induces a conformational change in NQO1. <p>(A) 16HBE cells were treated with DMSO or β-lapachone (10μM) for 2 h after which NQO1 was immunoprecipitated using anti-NQO1 antibodies. (B) Human cell lines (16HBE, ARPE19, TrHBMEC) were treated with β-lapachone (10μM) for the indicated times after which NQO1 was immunoprecipitated using antibodies that recognize the C-terminal domain of NQO1. (C) Intracellular levels of NADH and NAD<sup>+</sup> were measured by mass spectrometry in 16HBE cells treated with β-lapachone (10μM) for 2 h in the absence and presence of the PARP inhibitor olaparib (1μM). Results are the mean ± standard deviation, n = 3. (D) Immunoprecipitation of NQO1 from 16HBE cells treated with β-lapachone (10μM) in the absence and presence of olaparib (1μM) for 2 h. Reaction conditions are described in <i>Materials and methods</i>.</p> NAD;NQO 1 NQO 1;deacetylase Sirt 2;NQO 1 structure;Sirt 2-mediated deacetylation;NQO 1 co-localize;conditions NQO 1;NQO 1;pyridine nucleotides results;centriole duplication cycle NQO 1;acetyl α- tubulin;β- lapachone;FAD 2018-01-03
    https://plos.figshare.com/articles/figure/Intracellular_oxidation_of_pyridine_nucleotides_induces_a_conformational_change_in_NQO1_/5755239
10.1371/journal.pone.0190717.g004